FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4087887
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2512416.145986912064839No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1315213.2173345300396026No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA716551.7528615639326623No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT636101.5560606249634592No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA507831.2422799358201437No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC353390.8644808430370018No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT351200.8591235520942727No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA317880.7776144497144858No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA269740.6598518990373267No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA260410.6370283718703575No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC245070.5995028727555336No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT224640.5495259531391157No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT205050.5016038848431965No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC201900.4938981923913259No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA186220.4555409677420144No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC162750.3981274433466483No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA150430.3679896239793321No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC141700.34663384775557643No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT140240.34306232046042373No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA138800.3395397181967114No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA137840.3371913166875699No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC136450.3337910270024587No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT135320.3310267627260734No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC129820.3175723790799501No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA121510.2972440285164438No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC118670.2902966740519001No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC106160.25969406688589974No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA105980.2592537416029357No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC104210.254923876320456No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA101030.24714479632142475No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA100060.244771932296563No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA97130.23760441519053732No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT95810.23437536311546772No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA92710.22679198324219824No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC89530.21901290324316697No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA87980.21522121330653218No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87960.21517228827509174No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG87130.21314189947031315No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT83980.20543620701844253No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT81280.19883132777398202No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC81180.19858670261677977No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT79740.19506410035306748No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT78350.19166381066795632No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC75860.18557264425362052No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT73530.1798728780908083No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAT72590.17757340161310722No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC70940.17353708651927022No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA69650.1703814219913613No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA68760.16820425809226136No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66980.16384993029406145No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGTCG65900.16120797859627725No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC65430.1600582403574267No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG65020.15905527721289753No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA63380.15504342463478077No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGAGC62390.15262163557847858No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA62110.1519366851383123No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC61880.15137404727674714No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG60950.1490990333147663No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA59540.1456498185982147No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT57930.14171135356725859No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA57440.1405126902969676No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA56230.13755272589482048No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC55430.13559572463720254No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA53320.13043413382023525No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT52630.12874622023553978No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA51780.1266669063993207No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC51580.12617765608491624No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA51190.12522361797182752No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT49340.12069805256358603No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA48570.11881443885312876No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC48230.11798271331864114No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA47460.11609909960818389No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT46560.1138974731933637No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA46470.1136773105518817No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA45660.11169584677854354No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGAGAG44770.10951868287944358No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC44390.10858910728207506No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC43500.1064119433829751No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA41490.10149497772321005No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)