FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182225
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2436965.826946182952854No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA835491.997716526490086No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA436451.0435832601067614No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA435981.0424594563898402No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT391730.9366545319775956No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC369380.8832140786303941No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA337420.806795425879765No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA331880.7935488884505258No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA323020.7723639928506956No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT321680.7691599567216015No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA314250.7513942937072969No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC303470.7256185403702575No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT302430.7231318257626024No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC294780.7048401269659093No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC251490.6013306314222693No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA227680.5443992133373982No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT225860.5400474627740018No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT179580.4293886627333537No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC172860.4133206606531213No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA165380.39543544405191017No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC151990.3634189934783518No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC150740.36043015380568955No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA149610.35772824274160286No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC144250.344912098225227No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA137360.32843761394951254No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC128010.3060810931979987No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA123470.2952256275068893No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA121510.2905391269001548No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA119210.2850396619024562No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA114400.2735386068420518No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT113960.2724865352772747No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC111520.26665232023623786No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT108710.2599334086520931No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC105650.2526167291334158No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA104630.2501778359605234No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98300.23504235185816164No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT96670.23114490492501003No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA94570.22612365427493739No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA92430.22100676075533957No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTG90810.21713322453956926No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT89840.2148138849535833No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA84510.20206947258935137No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA83110.19872197215596962No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA81980.1960200610918829No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC81330.19446586446209851No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC81180.19410720370137904No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA78940.18875120300796824No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA78760.1883208100951049No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAA76590.18313218442336318No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76250.18231922003239903No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA71630.17127246860223924No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT71610.17122464716747665No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC70770.16921614690744757No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG68980.1649361284961952No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTT67800.162114663845202No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC64990.1553957522610572No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC64590.15443932356580528No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC63620.15211998397981935No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA63140.15097226954551704No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA62230.1487963942638189No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC59060.14121669685394736No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT57980.13863433937676714No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT55220.13203498137952885No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54680.13074380264093874No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT54600.13055251690188835No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC53820.1286874809461471No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT53780.1285918380766219No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT53780.1285918380766219No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT53480.12787451655518295No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA52350.12517260549109624No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA51780.12380969460036224No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50930.1217772836229519No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT50390.12048610488436179No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT49220.1176885509507499No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA47800.11429322908260554No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG47100.11261947886591467No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC45350.10843510332418749No Hit
CCCCGGCACCTCCACCACGCCTTGGCCGTACAGCA45290.1082916390198997No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC44610.10666571023797142No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT44420.10621140660772674No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA43910.10499196002128053No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC43460.10391597773912213No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT42860.10248133469624421No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC42710.10212267393552475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)