FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002406367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15708430
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC8426045.364024285049492No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2770361.7636135501765613No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2047751.3035994049055188No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2007151.2777534101116408No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1719121.0943932652722137No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1491650.9495856683322267No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA1204150.7665629219470055No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT1198100.7627114867622035No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN914210.5819868694707236No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA902930.574806011803853No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTGAA837090.5328922113794949No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT780450.49683513883946384No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA721520.45932025033692103No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN712980.45388367901820864No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA633110.40303836857025177No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA601730.3830618336778405No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC487260.3101901335779578No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA420140.2674614840566498No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA363160.2311879672252415No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGACTT299050.19037548628347964No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA291300.18544182964179107No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC285930.18202328303974363No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT265560.1690557235828151No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC252720.16088176857903685No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTCTT236970.1508553050814117No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA233340.1485444439705305No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC229570.14614445873967036No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC216250.13766493532453594No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC196420.1250411403303831No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA185950.11837592935767609No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA180880.1151483630127263No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC180380.11483006258423026No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT179080.11400248147014057No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT178810.1138305992387527No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA178540.11365871700736484No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA175090.11146244405074218No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC161680.10292562655847848No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA161260.1026582541985418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)