FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996028
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC675922.256053681741292No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA625362.087296914448063No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC535691.7880006461888875No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT462171.54260908109003No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA353691.1805296879735436No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT263200.8784964626498817No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT220060.7345058190377394No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC205110.6846064188986218No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT194540.6493263747868845No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC190250.6350074164860943No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA175660.5863096072533367No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT145040.4841076251623817No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC144570.4825388814790783No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA141410.47199158352325143No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA126610.42259284626178395No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA126570.4224593361610773No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT126250.42139125535542393No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA116790.38981611653829673No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA114980.3837747844813199No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC104740.3495961987004127No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC102320.3415188376076592No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC91630.3058382631938019No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA90160.30093176699283186No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA88880.2966594437702184No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT88800.2963924235688051No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG87240.291185529641245No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA85760.28624565591509826No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC85480.2853110852101516No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC81730.27279451326890136No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA79840.26648616101051126No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG78390.2616464198598945No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT77120.2574074741624578No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG76110.2540363441196144No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA72150.24081884414965415No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72130.2407520890993008No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC71980.24025142622165083No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA70800.23631287825080405No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA68320.22803525200699057No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT66410.22166014469824713No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA65190.21758808662669374No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA65170.21752133157634043No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC62370.2081756245268736No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA62280.20787522680028359No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC62270.2078418492751069No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGT60210.20096607908871347No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA60100.20059892631177015No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT58530.19535865485903336No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC57330.19135335183783328No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA57150.19075255638465327No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC56630.18901692507546658No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA53280.17783545414128304No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51810.172928957940313No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG50860.16975809304852957No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC49910.1665872281567462No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG48260.1610799365025961No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC47330.15797582666116605No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC46750.15603993020091936No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA46460.155071981970796No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA46390.15483833929455934No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT46080.15380363601408265No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG45780.15280231025878263No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA44320.14792919158298923No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC44210.14756203880604588No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT42760.1427222976554291No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT40790.13614692519562568No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC39260.1310401638435956No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC38410.12820307420357888No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC37530.12526585198803217No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA37460.12503220931179548No Hit
CTGACCTATGAATTGACAGCTATTCCATAATGCAC37000.12349684315366878No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGAATGC36900.12316306790190212No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA35810.1195249176576454No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG35050.11698822574421869No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA35040.116954848219042No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34910.11652094039174535No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC34590.11545285958609197No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC34500.11515246185950197No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT33740.11261576994607528No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT33240.11094689368724191No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC32700.10914450732770187No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA32560.10867722197522853No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGGTACA32500.10847695682416852No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA32390.1081098040472252No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA31970.10670794798980517No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA30950.10330344042178512No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC30790.10276940001895843No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA30540.10193496188954175No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA30290.10110052376012507No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA30080.10039959573141506No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)