FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986307
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1502115.029991892996936No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA968563.2433370045343626No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT620182.0767456259520536No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN377391.2637347734174684No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA364371.2201357730467766No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA355921.1918399548338465No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT331321.1094639633500507No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA327551.096839675224282No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA322441.0797282395949244No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN305811.0240407299048624No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT305011.0213618358728689No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC236770.7928521749438353No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA227480.7617435179973123No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA186830.6256222149966497No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT186270.6237469891742543No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG145450.4870564211917931No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143550.4806940478658088No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139650.4676344394598412No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA138800.46478811455084823No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC122450.41003821777198396No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC121160.4057185011453946No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT114480.38334973597825006No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT104560.3501314499815324No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGAA97920.32789662951598747No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA96430.32290718938139984No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA96230.32223746587340146No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA85920.28771321903608704No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGG84870.2841971706190958No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGAT84240.282087541568901No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80980.271171048388528No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGC80920.2709701313361285No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA80010.2679228893747361No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC77610.259886207278756No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT76430.2559348385815658No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA75510.25285411044477346No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA72250.2419376172644005No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT70200.23507295130741748No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA69430.23249451580162386No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA69040.2311885549610271No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA66990.22432388900404412No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA65070.21789454332726005No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC61600.20627484046348887No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA61570.20617438193728913No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA57770.19344963528532064No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA57080.19113908918272635No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT56330.1886276260277326No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC54790.18347075501614535No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC54440.18229873887714826No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC51270.17168362127537456No Hit
TCGATTCCCATCACCCGCTCCACGCCTTGGCCGTA50940.1705785774871773No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT50610.16947353369898005No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT49590.1660579438081885No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT47470.1589588746234061No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT44770.1499176072654285No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA43160.14452633302604187No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC42780.143253858360845No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG42630.14275156572984626No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC41630.13940294818985455No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG41040.13742726384125947No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC40930.13705891591186037No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA40520.13568598272046375No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA40480.1355520380188641No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA39600.1326052545836714No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC39150.13109837669067514No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC38100.12758232827368385No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT37780.1265107706608865No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA37720.126309853608487No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC37260.12476948954009083No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA36950.12373141810269339No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC35650.11937821530070417No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35230.11797179593390768No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG34400.11519244337571456No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA33920.11358510695651855No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC33640.11264749404532085No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC33450.11201125671272244No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC33240.11130804702932419No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32940.11030346176732667No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT32450.10866263917273074No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT31710.10618466219313688No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT31330.10491218752794003No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC31000.10380714373974277No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA30400.10179797321574774No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC30200.1011282497077494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)