FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587608
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN420671.172564003648113No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA411081.1458331010522889No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAGAC380501.0605952489792643No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN324740.9051713565138666No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC246570.687282445573764No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN228380.6365801391902348No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA209150.5829789653719135No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187320.5221306229666117No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA185970.5183676700464488No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT180630.5034831007178042No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT177740.49542759409612197No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT174370.4860341486583818No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA172850.4817973424075317No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT164150.4575472013664815No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA156060.4349973575708383No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA131590.3667903516772178No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA129800.3618009548423351No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA127950.3566443156554451No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAAGT122280.34083991339076064No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT100600.28040967686547696No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA94850.2643822847981162No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC86570.2413028402211167No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG84300.234975504570176No Hit
GCTCCCCGTCCTCCCCCCTCCCCCGCCTTGGCCGT83220.23196514223404563No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC76140.21223054469719102No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA73720.2054851031662322No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA70870.1975410914458882No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT61050.17016909316736947No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG59970.16715873083123908No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT57490.16024604694827305No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA56120.156427346577441No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT51120.1424904839101708No Hit
GCTCCCCGTCCTCCCCCCTCCCCGCCTTGGCCGTA50440.14059507058742204No Hit
GCTCCCCGTCCTCCCCCCTCCCGCCTTGGCCGTAC49430.13777982432863345No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT49370.1376125819766262No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA47830.13332002827510697No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC47710.1329855435710925No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA46940.1308392667203329No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC43960.12253289657063983No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT42820.11935529188250223No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA41740.11634492954637184No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC40720.11350180956224871No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT40420.11266559780221251No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGATCA40370.1125262291755398No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA39780.11088167938080191No Hit
CTCTCTCTCTCCCCCGCTCCCCGTCCGCCTTGGCC39450.10996184644476209No Hit
GGCTCACATCACCCCATAAACACCACGCCTTGGCC38850.10828942292468964No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGACTG37750.1052233131378902No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA37200.10369025824449048No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA36830.10265893040711249No Hit
ACACCTCTTTACAGTGACCACGCCTTGGCCGTACA36600.10201783472441806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)