FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162690
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC915992.896237064018288No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT639542.022139381349421No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA637552.01584726925497No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN548171.7332397421182602No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT496801.5708147178509435No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN463211.4646076599350553No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAC447811.4159149331739753No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA399021.2616475215718266No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT379671.200465426583067No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA343041.0846462979299267No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA337301.066497190682615No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA320891.0146109798936982No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG232430.734912368901157No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA221410.7000686124786178No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC221380.699973756517395No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215370.6809709456190775No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA179180.5665430377305395No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174190.5507653295138No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT158760.5019777467914971No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA125130.3956442142606452No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC120020.37948708219901417No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA119710.3785069039330443No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA118510.374712665484129No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA118140.37354277529571345No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA117680.37208831722362923No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT112750.3565003209293355No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT111610.3528957944028659No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA89390.2826391457904505No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA88360.27938242445513156No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT87940.2780544409980112No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA81790.25860896894732016No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA81350.25721774818271786No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT80420.2542772133848085No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT79080.2500403137835197No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT78660.24871233032639936No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAA78600.2485226184039536No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC70720.2236071192560763No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT70690.22351226329485344No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA68550.21674587139428775No Hit
CTGACCTATGAATTGACAGTTGTATTCCAATTACG67160.21235087852429418No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT66800.21121260698961958No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG66460.21013757276242695No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC65050.2056793425849514No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA63390.20043064606395186No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC63280.20008284087280134No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC60000.1897119224457661No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC59390.18778318456756748No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA57780.18269258131527275No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA55860.17662179979700826No Hit
CTGACCTATGAATTGACAGCCACGCCTTGTAATGC54910.17361802769161694No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA53310.1685590430930632No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA51300.16220369369113002No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC50070.1583145992809918No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA50060.15828298062725085No Hit
CCACTACGCCTTGGCCGTACAGCAGCATTCAACTC48080.15202248718654057No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG46460.14690026528050487No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT46440.14683702797302298No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA46400.14671055335805913No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT45830.14490829009482434No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA42780.13526460070383123No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT41790.1321343539834761No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA41660.1317233114848436No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT41430.1309960824488015No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA41070.1298578109141269No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA41030.12973133629916306No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG39620.12527310612168754No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC39520.12495691958427793No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC38590.12201638478636857No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA38240.12090973190543493No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC38000.12015088421565186No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG37310.11796919710752557No Hit
CGCCTTGGCCGTACAGCAGCATTCAACTCAGAGAT35980.11376391615997773No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC34980.11060205078588163No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA34460.10895788079135166No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG34000.10750342271926745No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA33940.10731371079682168No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC33860.107060761566894No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT33160.10484745580502675No Hit
CTGACCTATGAATTGACAGTTTATTCCAATTACGT33120.10472098119006289No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT32950.10418346407646654No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT32870.10393051484653885No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATA32380.10238120081323178No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG32060.10136940389352102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)