FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244119
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC828123.6901786402592736No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT515312.2962686025117205No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA398191.7743711452021929No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN272111.2125471064591493No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA255071.1366153042686238No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA225891.0065865491090267No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA216250.9636298253345745No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN204150.9097111160326169No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT172950.7706810556837672No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT170550.7599864356569327No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA151700.6759891075295027No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA151200.6737610616905788No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148140.6601254211563646No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC136760.6094150978624574No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA131210.5846837890504024No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN108320.48268385054446755No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC104570.4659735067525385No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA101680.45309540180355856No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT100200.4465003861203439No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT88870.396012867410329No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA86100.3836694934626907No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC76040.33884121118354243No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT75130.33478616775670095No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG72920.32493820514865746No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA64650.2880863269728566No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT61570.27436156460508554No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG60140.26798935350576325No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA59300.2642462364963712No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA53210.23710863817827843No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC53200.23706407726149994No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC51170.22801821115546902No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC48810.21750183479574836No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC47580.21202084203199562No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT46360.20658441018502138No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC43330.19308245240114272No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG43260.19277052598369337No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA42620.1899186273098708No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA41930.18684392405215589No Hit
CCCCGAGTGTTACAGCCCCCCCGCCTTGGCCGTAC41890.18666568038504197No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT41820.18635375396759263No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT40490.18042715203605514No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40350.17980329920115645No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA40300.17958049461726405No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC39670.17677315686022No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA37930.1690195573407649No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG37780.16835114358908773No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTG36900.16442978291258173No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC36870.1642961001622463No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA36600.1630929554092274No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT36370.1620680543233224No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA35820.15961720390050615No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA35210.15689898797701907No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA34380.15320043188440544No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC34280.15275482271662066No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT33730.15030397229380438No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA32670.14558051511528577No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32560.14509034503072252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC29960.1335045066683184No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC29250.13034068157704648No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA28300.12610739448309113No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA28090.1251716152307431No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA27750.12365654406027488No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC27250.12142849822135102No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC27030.12044815805222449No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC27010.12035903621866754No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA27000.12031447530188907No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT26610.11857659954752846No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA26600.11853203863074997No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC26450.11786362487907281No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC26270.11706152837706021No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA26240.11692784562672479No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC25890.11536821353947807No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC25870.11527909170592113No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC25560.11389770328578833No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA25520.11371945961867441No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG25480.11354121595156051No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG25300.11273911944954791No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC25250.11251631486565551No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA24910.1110012436951873No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGACAC24770.1103773908602886No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT24710.11011002535961772No Hit
CCCCGAGTGTTACAGCCCCCCCCGCCTTGGCCGTA24280.10819390593814321No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT23950.10672339568445345No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA23550.10494095901331436No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG23360.10409430159452329No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG23160.10320308325895375No Hit
CGCCTTGGCCGTACAGCAGATGTGAGAACAGAGTC22540.10044030641868813No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG22510.1003066236683527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)