FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3161192
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1575444.983689696797917No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA840522.6588704513993457No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT754302.386125233772577No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN517161.6359651675697016No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN392601.241936585946061No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC389171.231086248478422No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA362841.1477948824367517No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA334551.0583033235564305No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT310050.9808009130732964No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA308240.9750752247886241No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN280830.8883674259583094No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT276140.8735312502372522No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA255530.8083343245206239No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC228020.7213101893209903No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA213200.674429139387927No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC210660.6663941956072266No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206750.654025443566857No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGAT184350.5831660968394201No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC179690.5684248220291587No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGGC158990.5029431935801432No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC158750.5021839862937778No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA155150.49079587699829685No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT150220.47520049399087433No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA146920.4647613938033502No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC146070.46207253466413933No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA142770.4516334344766152No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT140960.4459077461919428No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA140330.4439148270652336No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA139900.4425545806771623No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT137220.4340767659794154No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA116380.3681522666133535No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC113180.35802950279514817No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC107260.33930238973146837No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA101510.32111304849563077No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA95470.3020063317887683No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA95370.30168999541944935No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA92420.2923580725245414No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA92390.2922631716137457No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA88820.2809699632290604No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC87580.2770473922495059No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC86970.2751177403966605No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC86480.27356769218699784No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA80790.2555681527727516No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC78630.24873528719546295No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT77560.2453504880437506No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG74850.23677777243520798No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA66930.21172393198514988No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA65970.2086871028396883No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG65440.20701052008229806No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC65370.20678908462377485No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA64620.20441656185388296No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG63700.20150626725614892No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA63150.1997664172248949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC62040.19625508352545495No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61290.1938825607555631No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT60270.19065592978851015No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA60050.18995998977600856No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG59820.18923241612657504No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT56360.17828717774814057No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC53380.1688603539424369No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC53170.16819604756686718No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC52860.16721540482197855No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA52380.16569699024924775No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC51410.16262852746685427No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50490.15971823286912024No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG49990.15813655102252566No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC49480.15652323553899922No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC48400.15310680275035493No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT48380.15304353547649113No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT48070.1520628927316025No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA47730.15098734907591818No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGTA46670.1476341835611377No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC45810.14491369078499503No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA45140.14279423711055828No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC43690.13820735975543402No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG43110.1363726088133843No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT42840.13551850061622325No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC42430.1342215215020157No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC42300.1338102842219011No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT42120.13324087875712706No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG41530.13137449417814545No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG40960.12957137687302764No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC40660.12862236776507088No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT39980.1264712804537023No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC39930.12631311226904282No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA39560.12514266770256285No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC39130.12378242131449149No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA39120.1237507876775596No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA38610.12213747219403315No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA38600.12210583855710125No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT38420.12153643309232719No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT36740.11622198208776943No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA36580.11571584389685917No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA35760.11312188566844407No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35490.11226777747128298No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT35130.11112896654173489No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCGC34710.10980035379059545No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC34570.10935748287354896No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG33760.10679515828206576No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC33660.10647882191274685No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA32910.10410629914285496No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA32400.1024929836593285No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA31730.10037352998489178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)