FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278060
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC767522.341384843474494No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN520261.5870972465421622No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN381471.1637065825518753No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT369241.1263979304832736No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA368391.1238049334057338No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN312240.9525145970482543No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA306840.9360414391438839No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN236000.7199380121169229No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA226240.6901643044971721No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA226200.6900422811052879No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT225920.6891881173620983No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA215090.6561502840094445No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA202620.6181094915895377No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA200670.6121608512351818No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT199970.6100254418772079No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT190530.581227921392531No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA189470.577994301507599No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA175150.5343099272130467No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC173450.5291239330579672No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA156820.478392707882101No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA142750.43547097978682514No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC139550.42570910843608717No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT124950.38117057039834534No Hit
CCCCGAGTGTTACAGCCCCCCCGCCTTGGCCGTAC119790.36542955284528045No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC113010.34474658792090446No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGAGAG112510.34322129552235164No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA100340.30609567854157643No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC97610.29776758204547815No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC94950.2896530264851772No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA91400.2788234504554523No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT91250.2783658627358865No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC90320.27552881887457825No Hit
TCGAGTCCACTTAAGCCCACCACGCCTTGGCCGTA80990.24706686271758296No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC79200.2416063159307639No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG77480.23635931007974226No Hit
TCGATTCCCATCACCCGCTCCACGCCTTGGCCGTA74830.22827526036741244No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC72750.22193004398943275No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA70180.21409004106087134No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT69120.21085642117593942No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG67170.20490778082158348No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA64610.19709828374099314No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG63630.19410871063982965No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT61650.18806855274156056No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATCT60820.1855365673599629No Hit
TCGATTCCCGTACTGGCTACCACGCCTTGGCCGTA59750.1822724416270599No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC59010.18001500887720176No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA57220.17455446209038272No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG56950.1737308041951642No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC55860.1704056667663191No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT55700.16991757319878223No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG55060.1679651989286346No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC53780.16406045038833944No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT53760.16399943869239733No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA53390.16287072231746827No Hit
TCGAATCCTCTTGCCCACGCCACGCCTTGGCCGTA53310.1626266755336998No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA52240.1593625498007968No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA51590.15737966968267816No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA50860.15515274278079108No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC49800.15191912289585913No Hit
TCAAGTCCCATCTTCCGCACCACGCCTTGGCCGTA46720.14252332172077387No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG46020.14038791236279996No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA45780.1396557720114946No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAACA45760.13959476031555249No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT44340.13526292990366254No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGTG43840.13373763750510972No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA43520.13276145037003595No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC43000.13117514627554103No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA42260.12891771352568288No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA41780.12745343282307217No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA40910.1247994240495903No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA40670.12406728369828496No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT39800.1214132749248031No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC38950.11882027784726332No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG38610.11778307901624742No Hit
TCGAGTCCAACAGGGGGAGCCACGCCTTGGCCGTA38550.11760004392842108No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC38100.11622728076972355No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA38080.11616626907378146No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA38060.11610525737783933No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT37960.11580019889812877No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG37330.11387833047595225No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG36710.11198696790174675No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG36680.1118954503578336No Hit
TCGAATCTCTTCATCTCCACCACGCCTTGGCCGTA36480.11128533339841247No Hit
GTTCGAGCCTCTCCTCCCGTGCCACGCCTTGGCCG35560.10847879538507532No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC35330.10777716088174102No Hit
TCGAGCCCTATAGGTCCCACCACGCCTTGGCCGTA35080.10701451468246462No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG33330.10167599128752983No Hit
TCGAATCCTGTCACCCCGACCACGCCTTGGCCGTA32850.10021171058491912No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATAG32830.10015069888897701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)