FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302918
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT867372.626071855250418No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA610051.8470031650800898No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC594761.8007107654504289No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA417851.2650934718936409No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA399051.2081741054425208No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT396101.199242609111095No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA370471.1216445579333185No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA318040.9629061333039451No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT315960.9566086714838212No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA259950.7870313462217348No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGC235410.712733407247773No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA230900.6990788145512543No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA195230.5910833995878796No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA176740.5351025971580281No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC155220.4699480883267462No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT144790.438369950449875No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC136370.4128773405818733No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGG128530.38914075372140633No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG119040.36040858416709104No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC117540.3558671453545017No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC85780.2597097475626098No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC85070.2575601331913175No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT84890.25701516053380674No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG82770.2505965936786805No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT79340.24021183692722617No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA78950.23903106283595293No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT75260.22785912335698313No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG74470.22546729891568607No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA68830.2083914889803501No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA68660.20787679258158998No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA66080.2000655178239363No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT63980.1937075034863112No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT59980.1815969999860729No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG59110.17896296547477109No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC58480.17705556117348356No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA56790.17193887344463288No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA56590.171333348269621No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53500.1619779843156869No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT52930.16025223756690296No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA51630.1563163239293255No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50680.15344007934801893No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50070.1515932275642326No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG50030.1514721225292302No Hit
GCATTGGTGGTTCAGTGACGAGGCCTCTATTCTAT49040.14847477291292124No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT48690.14741510385665038No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA48460.1467187499053867No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC48330.14632515854162895No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA46970.14220758735154795No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC46310.14020935427400863No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC45280.13709089962269724No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC45160.13672758451769013No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAC42880.12982459752255432No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42810.12961266371130015No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC42660.1291585198300412No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC42150.12761443063376082No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT41170.12464735727620244No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40310.12204359902365121No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC39990.12107475874363215No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC38800.11747188395231127No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37730.11423232426599753No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG37680.11408094297224454No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36970.11193132860095226No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC36830.11150746097844391No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT36670.11102304083843437No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC35260.10675408835460037No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT34420.10421088261955035No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC34230.10363563370328903No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCATGCA34200.10354480492703726No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA33740.10215209702450984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)