FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988716
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC503341.6841345915771189No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT378191.2653928978196658No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA325951.0906021181002141No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA297410.995109605596517No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT252710.8455470509743984No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA204370.6838053532018432No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA202520.6776154040731873No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA181360.6068157697151553No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT157060.5255099514306477No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT129120.4320249899957039No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124940.41803905088338944No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT122490.40984155068598016No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG117670.3937142237669956No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA114910.3844794888507305No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA108710.36373479447361345No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA102660.3434919878636846No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG96050.3213754669229194No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA82720.2767743740121176No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGCTTG79050.2644948533082434No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC77260.2585056592864628No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT75150.25144577136134716No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA74410.24896979170988479No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA72740.24338210790185483No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGAGGT72560.24277984258122887No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA71060.23776096490934567No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC70550.2360545465009054No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA65690.21979338284400393No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT65210.2181873419890013No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA64790.21678205624087402No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA64080.21440645414284928No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG63310.21183009693794924No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG57170.19128615766770746No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG52710.17636336138997483No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC52230.17475732053497223No Hit
CGCCTTGGCCGTACAGCAGGACCGCATCCAGTCCA50240.16809894282360718No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA49970.16719554484266821No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC49960.167162085658189No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT49590.16592409583245782No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA49070.16418421823953833No Hit
TATTGCACTTGTCCCGGCCTGTCGCACCAATTACG48680.16287931004484868No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA47210.15796080992640318No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA45410.15193815672014335No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG45050.1507336260788914No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT41660.13939096254043543No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC41650.1393575033559562No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC40820.13658039104418085No Hit
GCTCCCCGTCCTCCCCCCTCCCGCCTTGGCCGTAC40450.13534240121844968No Hit
TACCCTGTAGATCCGATAAACAGCGGAACCGGCAT40370.1350747277426159No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC40250.13467321752886524No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA39390.13179572766365222No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA38590.12911899290531453No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA36100.12078765596998844No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC34780.11637104361873125No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA34740.11623720688081438No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT34020.11382814559831045No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC31950.10690209441111168No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT31870.10663442093527789No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC31160.10425881883725319No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC30420.10178283918579083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)