FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595528
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN322761.2435234757629277No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN239630.9232418220878372No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN156770.6040004191825324No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC148430.5718682287380449No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN117520.45277877950074125No Hit
CTGACCTATGAATTGACAGCCACGCCTGATAATCG102590.3952567647122281No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC90940.3503718703862952No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCGC78550.3026359183950241No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT70500.27162103433289875No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC69960.2695405327933276No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC68720.26476308481357164No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC68700.26468602920099493No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG67280.2592150807080486No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG64700.2492749066856532No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGCCGT53500.2061237636426962No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGACGCCT51370.19791734090327673No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC46270.17826815969621596No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC43770.16863620812412736No Hit
CTGACCTATGAATTGACAGCCACGCCTGATAATGC41360.15935100680863393No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCCGGCA40950.1577713667508114No Hit
CTGACCTATGAATTGACAGCCCGAAGGCTAATGAC40390.15561380959866353No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAATTACG40200.1548817812791848No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT38010.14644419170203518No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG35350.1361957952293329No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC34770.13396118246460836No Hit
CTGACCTATGAATTGACAGCCCGCCTTAGCCGTAC32460.12506125921199848No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTCGGCCGT32320.12452186992396153No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTCTTAATG30960.11928208826874531No Hit
CTGACCTATGAATTGACAGCCCGAAGGCTAATGCA29540.113811139775799No Hit
CTGACCTATGAATTGACAGCCCGCCTTAGCCGTCA29200.11250119436199493No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTATAATGCA28180.10857135812058279No Hit
TGACCTATGAATTGACAGCCACGCCTGCGCCGATG27910.10753110735079721No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTCGC27100.1044103550414405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)