FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720237
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1660132.9022049261245644No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA795371.3904493817301626No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN789431.3800651965993715No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA688761.20407598496356No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC610281.0668788723264437No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN509570.8908197335180343No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT484170.846415978918356No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA453800.7933237731233863No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA413050.7220854660392567No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA410710.7179947264422786No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT390360.6824192773830874No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC385550.6740105348781877No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT371730.6498506967456069No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA367000.641581808585903No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT342090.5980346618505492No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN324750.5677212325293515No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN298580.5219713798571632No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA287320.5022868807708492No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC276360.4831268354790195No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC273240.4776725160163819No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT269430.4710119528264301No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC231810.4052454469980877No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA231050.40391683071872725No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA221650.3874839451582163No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA201080.3515238966497367No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA198210.34650662201583604No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC195570.34189142862437344No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT195100.3410697843463479No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT189720.33166457963192786No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA177730.31070390964570177No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA177630.31052909171420695No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT164960.28837965979381625No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA163860.28645666254737345No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA163500.2858273179939922No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA163210.28532034599265726No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG159110.27815281080137066No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA158290.2767193037631133No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC152960.2674015080144407No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC150160.2625066059325864No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC144240.2521573843880944No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT140170.24504229457625618No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA137740.24079421884093266No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC135100.23617902544947No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA130770.22860940901574534No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA129470.22633677590631296No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG125670.21969369450951073No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA119360.20866268303218904No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA117520.2054460330926848No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG117050.20462438881465925No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG114270.1997644503191039No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC113610.19861065197123826No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC107550.1880166853226536No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG103170.1803596599231815No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT100470.175639575772822No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC98340.17191595383198285No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA95270.16654904333509257No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG94320.164888272985892No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC93060.16268556704905757No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT91300.15960877145474917No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC89600.15663686661933762No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA85720.14985393087733953No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC84650.1479833790103452No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT82690.14455694755304718No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA79490.1389627737452137No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT78710.13759919387955427No Hit
TCCCTGAGACCCTTTAACCTGTGACGTTCCAATTA78110.1365502862905855No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA76890.13441750752634898No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG74600.13041417689511817No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC72280.1263584008844389No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC71470.12494237563933103No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG69110.12081667245605385No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTG66940.11702312334261673No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA66720.11663852389332821No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC65830.1150826443030245No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT64600.1129323837456385No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCACA63870.1116562128457265No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA63090.11029263298006708No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT61480.10747806428300086No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG61430.10739065531725346No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA60720.10614944800364041No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA59290.10364955158326482No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT58860.1028978344778372No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA58290.10190137226831686No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA58210.101761517923121No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG57320.10020563833281733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)