FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510054
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT567462.2607481751388616No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA351891.4019220303626934No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265491.0577063282303887No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT262361.0452364769841607No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246850.9834449776777711No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC238470.9500592417533646No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA223660.8910565270707324No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA211040.8407787242824257No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179780.7162395709415017No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA151780.6046881859912178No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127170.5066424865759861No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT124910.4976386962192846No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA122120.48652339750459556No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC120970.48194182276556596No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT110700.4410263683570155No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA108930.4339747272369439No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA100940.4021427427457736No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA89200.3553708406273331No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA76170.3034596068451117No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA75920.302463612336627No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC68190.2716674621342808No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG65060.2591976108880526No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC59270.2361303780715475No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA54630.2176447199940718No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC54160.21577225031812064No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT49390.19676867509623297No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG48980.1951352441023181No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT48480.19314325508534877No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC47170.18792424386088905No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT46210.1840996249483079No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC46030.1833825089021989No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA45600.18166939834760526No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT44610.17772526009400594No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT44440.17704798382823636No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCG41180.16406021543759616No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC40980.16326341983080842No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT39630.15788504948499116No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA39630.15788504948499116No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC38660.15402059079207062No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC37050.1476063861574293No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC36590.14577375626181746No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA35130.13995714833226694No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG34370.13692932502647354No Hit
CTGACCTATGAATTGACAGCTTATGAACCGGCATG33820.13473813710780724No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC33630.13398118128135889No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA33140.1320290320447289No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA32200.1282840926928265No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT32040.12764665620739632No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC31850.126889700380948No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA30800.12270652344531234No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT30660.12214876652056092No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC29680.11824446804730097No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATA29670.1182046282669616No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA28930.11525648452184695No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA28700.11434016957404104No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA27830.11087410868451436No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA26820.10685029087023626No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC26690.10633237372582423No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA26550.1057746168010728No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG26280.10469894273190934No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC25770.10266711393460061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)