FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5037654
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1439362.8572029758296225No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN594161.1794378891444313No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA539661.071252610838299No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC447160.8876353953645882No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN428280.8501576328981705No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN394650.7834003685048635No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT359940.714499249055215No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA350230.6952244040579206No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC341370.6776368523920063No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA333480.6619748001748433No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT288120.5719328878084918No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT284820.565382219580781No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA261100.5182968103803874No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA239880.4761740286252291No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA238150.47273989043312625No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232900.4623183727981318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC196040.3891493937455808No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGAGAG194140.38537779688720186No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA186590.3703906620025909No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA176850.3510562654759537No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATCT170230.3379152280009703No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA167830.33315110565354433No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC166650.33080874549939315No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA166350.33021323020596494No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC165470.32846638534524203No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC160670.31893814065039No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT159890.3173898008874766No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA153990.30567800011672097No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC151390.3005168675736762No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT150030.2978171982434681No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT132940.26389267702783875No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA125660.2494415059073132No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC120030.23826566890064305No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG108000.21438550563417016No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA105130.20868840932703994No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC103520.20549247725230835No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC102540.20354712729377603No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC100590.1996762778864924No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT100210.1989219585148166No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC99880.19826689169204553No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG97470.1934829188348386No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC92470.18355766394436776No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC92290.1832003547683108No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA92180.18298199916072044No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA85640.16999976576398457No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA82820.16440192200575904No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTAA77530.1539010023316409No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA77420.15368264672405052No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA76000.1508638743351568No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC72450.1438169433629225No Hit
CGCCTTGGCCGTACAGCAGAACCCTAGCGAGATAG68590.136154646587479No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA68530.13603554352879338No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA68080.135142270588651No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC67470.13393138949201353No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG66360.13172798290632903No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC66300.13160887984764336No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT66290.13158902933786243No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG65590.13019949365319652No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC65410.12984218447713955No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG64740.12851220032181646No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA64340.1277181799305788No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT64010.1270631131078077No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA63030.12511776314927545No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG62710.1244825468362853No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA62690.12444284581672344No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG59900.11890455358784069No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA59170.11745546637383195No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC57690.11451759092625258No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC57500.11414043124041467No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC56950.11304865320246289No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC56320.11179807108626356No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA56080.11132165885152097No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG52480.10417547533038198No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT52450.10411592380103914No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG51830.10288519219462074No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT50560.10036417745244117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)