FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825576
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1827636.46816790629592No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA700382.4787158441323114No Hit
CTGACCTATGAATTGACAGCCCGCCTTCGCCGTAC409591.4495805457011244No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA374201.3243317468721423No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT349751.2378007174466374No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN332581.1770343462713444No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA325431.151729771204172No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA321051.1362285070371494No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN266830.9443384286956005No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA246480.8723177150428798No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT206660.7313906969764749No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC194230.6873996664750833No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA190080.6727123956318996No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA164140.5809081051084806No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT157820.5585409842099451No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGCCGTA155220.5493393205491552No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142170.503154047174806No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA119950.42451521388913266No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA113460.40154644575123793No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA110580.39135383369620924No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG109000.38576205347157533No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN107990.3821875610494993No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA99010.35040643040569425No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA95190.33688706302714916No Hit
CGCCTTGGCCGTACAGCAGCCTTTAACGAAGAGAA90660.3208549336489268No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT85740.303442554721586No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCGCCGT85500.3025931703836669No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80880.2862425218787249No Hit
TGACCTATGAATTGACAGCCAGCGCCTTCGCCGTA80460.28475609928736656No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC78440.2776071144432144No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT75280.26642355399394674No Hit
CTGACCTATGAATTGACAGCCGCCTTCGCCGTACA74620.2640877470646693No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA69480.24589676582756934No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTCGCCGTA68700.24313626672933236No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT68610.2428177476026127No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA68380.2420037542787736No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC67030.23722596737797888No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT63660.22529919563303197No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT62030.21953046033799833No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA61870.21896420411271897No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA61780.2186456849859993No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTCGCCGTA59560.21078887986024797No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC55600.1967740382845834No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTCGCCGTA53420.18905879721515187No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA49670.1757871669351665No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC49010.17345136000588907No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC48480.17157563625965114No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTCGCCGT47370.16764723369677545No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA46950.16616081110541708No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC46840.16577150995053752No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA46350.1640373502606194No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG45580.16131224217646242No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA45090.15957808248654434No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA41840.148076002910557No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC41180.14574019598127957No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA40980.14503237569968033No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC40870.1446430745448008No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT39380.1393698134468866No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT39140.13852042910896753No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTCGCCGTA38200.1351936737854512No Hit
TGACCTATGAATTGACAGCCAGACGCCTTCGCCGT37320.1320792645464146No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA37080.13122988020849555No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA36460.12903563733553794No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC35720.12641670229362084No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT34710.12284220987154477No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA34340.12153274235058621No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT34120.12075414004082707No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC32980.11671956443571152No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC32310.11434836649235412No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG32090.11356976418259498No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA32060.1134635911403551No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGCGCCGT31970.11314507201363544No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG31870.11279116187283583No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC31150.11024300885907863No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC30850.10918127843667982No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT30510.10797798395796114No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC30010.10620843325396309No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG29770.10535904891604402No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA28480.10079360809972905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)