FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123976
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1406326.62116709416679No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT596722.809447941031349No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA510532.403652395318968No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT485782.287125654903822No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA357131.6814220123014572No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN344051.6198393955487258No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297911.4026053025081262No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA285191.342717620161433No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA233491.099306206849795No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA191570.9019405115688689No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA173990.8191712147406561No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151610.713802792498597No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA151090.7113545539120969No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141570.6665329551746347No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC139160.6551863109564326No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC133210.6271728117455188No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA129850.6113534239558263No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT123490.5814095827824797No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA122990.5790555072185374No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA96940.45640817033714126No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA93780.4415304127730257No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA91040.4286300786826217No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT87240.4107391043966599No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC85570.40287649201309245No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA77880.3666708098396592No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT76750.36135059906514955No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA71520.33672696866631263No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC63140.297272662214639No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC60480.2847489802144657No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA59120.27834589468054255No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC53530.2520273298756671No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC53060.24981449884556134No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA50430.23743206137922462No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC50050.23564296395062845No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48200.22693288436404177No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA47440.2233546895068494No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC46670.2197294131383782No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG46400.21845821233384935No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC41490.19534119029593558No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC40420.1903034685890989No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA40300.18973849045375277No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC40190.18922059382968545No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC38020.17900390588217568No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG37720.17759146054381028No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC36750.17302455394976216No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA36300.17090588594221404No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA35210.16577400121281974No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA34790.16379657773910816No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA34150.16078336101726196No Hit
CGCCTTGGCCGTACAGCAGCTAAGACGACAGAGTG33880.1595121602127331No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT33790.15908842661122347No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC33720.15875885603227155No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA33190.15626353593449266No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG32840.15461568303973303No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA31950.15042542853591567No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG30400.1431277942876944No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG29470.13874921373876165No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT29430.13856088769364627No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC29300.13794882804702124No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG29100.1370071978214443No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC28510.13422938865599235No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA28440.1338998180770404No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG28410.13375857354320386No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA28250.13300526936274232No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG27280.12843836276869416No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA26540.12495433093405951No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA26430.1244364343099922No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT25780.12137613607686715No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA25680.12090532096407867No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA25350.11935163109187674No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA25190.1185983269114152No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT24050.11323103462562666No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA23620.11120652964063624No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA23050.10852288349774196No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA23010.10833455745262659No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT22710.10692211211426117No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC22380.10536842224205924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)