FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066528
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1142833.7267880808523515No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN614882.0051341451961306No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT413431.3482022665372695No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN354791.1569762284903318No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA341301.1129851088918803No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA331051.079559684437905No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA326111.0634502603595988No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA298930.9748158177587161No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT278100.9068888332341984No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA249960.8151238142942117No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN248370.8099387972325706No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC238130.7765459829487943No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGGC211150.6885637437518913No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA211080.6883354725604983No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA207930.6780632689478132No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT173150.5646450969956902No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC171000.5576339104029051No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA156630.5107730958269417No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA137030.44685716223690114No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA128370.41861675484456695No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA122280.3987571611933757No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116970.3814411608177065No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA116150.3787671268613885No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC113550.3702884826096484No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC111940.36503824520760936No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC99680.32505817654363506No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA99540.32460163416084903No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC99180.323427668033685No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT99140.32329722735288896No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA97360.31749261705746695No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGAT97180.31690563399388494No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA91000.29675254881090274No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA89830.29293715889761973No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG84930.27695817550010954No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT79700.25990305648603246No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT77470.25263098853165533No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC72660.2369454966659362No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC71340.23264095419966818No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA69790.22758637781882313No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC63970.20860725876300493No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT63460.20694414008285592No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA59170.19295437706748478No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG56090.1829104446461927No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA55580.18124732596604368No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54700.17837763098853165No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA53060.17302956307589562No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA53050.1729969529056966No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC52790.1721490884805226No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA52040.16970332571559757No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC50150.1635400035479865No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA49880.1626595289526135No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA49560.1616160035062455No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT47780.15581139321082343No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC47140.15372434231808743No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC46360.1511807490425654No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGTA45750.1491915286604264No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG45660.1488980371286354No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA44510.14514786755575035No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC43050.1403867827066963No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT42820.1396367487921193No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA41290.13464739275167226No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA39200.1278318671800812No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC38600.12587525696814117No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT38450.12538610441515619No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT37860.12346210437341515No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC37830.12336427386281815No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC36490.11899451105615211No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36130.1178205449289881No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA36010.1174292228866001No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA35230.11488562961107807No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG34400.11217898548456103No Hit
AGCAGCATTGTACAGGGCTATCACGCCTTGGCCGT34190.11149417191038202No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC34070.11110284986799403No Hit
CAAAGTGCTTACAGTGCAGGTCGCCTTGGCCGTAC33240.10839620574147701No Hit
TCGAGGAGCTCACAGTCTAGTCGCCTTGGCCGTAC33220.108330985401079No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG33180.10820054472028301No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC33060.10780922267789499No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA32260.10520040906197498No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA32070.10458081582819397No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC31960.10422210395600498No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA31930.10412427344540798No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA31830.10379817174341796No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC31820.10376556157321896No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT31580.10298291748844296No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC31360.10226549374406495No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA31100.10141762931889095No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA30840.10056976489371693No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT30680.10004800217053293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)