FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366755
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1034473.0726025505271393No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1024173.0420092938155583No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA935242.7778677094115847No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA522141.5508701999402985No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA370631.1008523043702318No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA356821.0598335786239272No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA321050.953588841480892No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA312890.9293518536394837No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT285110.8468391670911605No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA284860.8460966123166075No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC220800.6558243768851609No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA204330.6069048683376129No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT198940.5908953873982514No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA171450.5092440643884096No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC149760.4448200121481961No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA119120.35381249897898714No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA114910.3413078765755156No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGACCA112330.3336447113021292No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGTGGT111400.3308824075407923No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC106590.3165956536783936No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT97580.28983397960350543No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC91770.272577006642895No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC90630.2691909568709336No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG90360.2683889977144164No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG87450.25974566013862016No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT82010.24358766824434808No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT79140.23506313943248025No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC76870.22832074207953948No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA76460.22710295224927265No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA75250.2235089871404364No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA73050.2169745051243705No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72270.2146577342277653No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG70890.21055883187223304No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA69630.20681635580848623No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT69150.20539065064134457No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC68880.2045886914848274No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC68790.20432137176598833No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68430.20325209289063206No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT67270.1998066387367064No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGACCC65870.19564833199920995No Hit
CGCCTTGGCCGTACAGCAGGGCATAACAACTGTTG65760.1953216078984066No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65260.19383649834930075No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG65030.19315334795671202No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT64690.19214347346332003No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA61880.1837971577973449No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG59950.17806463493779617No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC59780.1775596976911002No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC59450.1765795253886903No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC59390.1764013122427976No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA58670.17426275449208511No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC57700.17138164196681968No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT57210.1699262346086959No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA56670.16832231629566155No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC55560.16502537309664647No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA54000.1603918313034361No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT53130.15780774068799183No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA52960.15730280344129585No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC52910.15715429248638527No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA52800.15682756838558196No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT51620.15332270984969204No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA51580.1532039010857636No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50810.1509168323801405No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT49510.14705554755246522No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA47830.14206557946746942No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA44660.13264998492613808No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44640.13259058054417383No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44190.13125398194997856No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA44150.13113517318605006No Hit
CGCCTTGGCCGTACAGCAGGGCATAACAACTCAAC43960.13057083155738983No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC43750.12994708554676535No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGTGGG42850.12727388835837478No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC38530.11444254185409987No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA38350.11390790241642174No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA37560.11156142932883444No Hit
CTGACCTATGAATTGACAGCCACGCCTGTTAATGC37220.11055155483544245No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT37130.11028423511660337No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36900.10960108472401467No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG36790.10927436062321137No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCTAC35610.10576950208732146No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC35310.10487843635785794No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC34980.10389826405544805No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG34590.10273987860714545No Hit
GCTAAACCTAGCCCCAAACCCACCGCCTTGGCCGT34570.10268047422518121No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA34270.10178940849571769No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA33840.10051221428348661No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC33790.10036370332857604No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33770.1003042989466118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)