FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880492
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1156512.9803179596814013No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT663941.7109686091351304No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA654291.6861006284770077No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA521401.3436440533829217No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA429851.1077203612325448No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA386730.996600431079358No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA377530.9728920971876762No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT368520.949673391930714No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA288820.744287064629949No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA263710.6795787750625436No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA255670.6588597528354652No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA207130.5337725216287007No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC187370.48285114361787107No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA183770.4735739694863435No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT174040.4484998294030757No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA160700.41412274526013715No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC155590.40095431197899645No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG142110.36621644884205407No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT132480.3414000080402176No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT128850.3320455241242605No Hit
TCCCTGAGACCCTAACTTGTGACGCAGGTTAATGC124810.32163447315443505No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC118690.30586327713083805No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC117680.30326051438838164No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN115070.2965345631430241No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG112810.2907105593826762No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC110780.28547926396962037No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA109690.2826703418020189No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC107950.2781863743051139No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC96340.2482674877309372No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT92420.23816567589882934No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT89690.23113048551575416No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG83210.21443157207900443No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC78500.20229393592358907No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCTAC77210.198969615193125No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT72130.18587849169641374No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66240.1707000040201088No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA62860.16198976830773007No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA62520.16111359075086357No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61640.15884583707426791No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG61630.15882006714612476No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA59380.15302183331392No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT57200.14740398897871715No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC55600.14328080047581596No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC54910.14150267543393982No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGATG54600.14070380766150273No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGTAC54350.1400595594579244No Hit
ACAGCAGGCACAGACAGGCAGCGCCTTGGCCGTAC53970.13908030218848538No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC52670.13573021152987816No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG52540.13539520246401746No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA52260.13467364447600974No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT52060.1341582459131471No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT51800.13348822778142566No Hit
ACAGCAGGCACAGACAGGCAGACGCCTTGGCCGTA49140.12663342689535245No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC48990.12624687797320547No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47990.12366988515889223No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG47790.12315448659602957No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47390.12212368947030429No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC47310.12191753004515923No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCAGCCGT47170.12155675105115536No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT46720.12039710428471441No Hit
TAGCTTATCAGACTGATGTTCTGGCTTGGTCGGCA46440.11967554629670671No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC45890.11825820024883443No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC45540.11735625276382479No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT44490.1146504103087959No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC44490.1146504103087959No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG44430.1144957907399371No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA43770.11279497548249036No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA43510.11212495735076893No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA42710.11006336309931834No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT41190.1061463340215622No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA40960.10555362567427017No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC40900.10539900610541136No Hit
TAGCTTATCAGACTGATGTTCTGGCAACCACGGCA40590.10460013833297427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)