FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665536
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT2410366.575736809023292No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA1572024.288649736355065No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA1361203.7135087474246604No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT982282.679771798721933No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN858232.3413492596989904No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT683251.863983875755142No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA442661.2076269336871879No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA312230.851799027481929No Hit
CCACTACGCCTTGGCCGTACAGCAGGGAACCGCAC308300.8410775395467402No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA287640.7847147047525929No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA279850.7634626968607048No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN266540.7271514998079408No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG261050.7121741540664176No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN252990.6901855554003562No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA244240.6663145580891854No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN244120.665987184411775No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT239870.6543927000034919No Hit
ACAGTAGTCTGCACATTGGTTACGCCTTGGCCGTA218370.5957382494674721No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG190980.5210152075985612No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA185720.5066653280720746No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC173690.4738461169116877No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT150620.4109085274295492No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA145840.39786814261270387No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA142830.38965651953766106No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC141850.3869829678388099No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA136830.3732878356671439No Hit
CCACTACGCCTTGGCCGTACCTACTTTCCAATACA134030.36564911652756926No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT130800.3568373083772741No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA129540.35339988476446554No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA126000.34374236128086044No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC125320.34188724377553514No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT124340.339213692076684No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT116910.3189438052170269No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA114590.31261458078709364No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA108380.29567299298110833No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT105620.2881433984006705No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC100050.27294780354087367No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA99460.27133821629360616No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT98130.2677098247023082No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA97730.2666185791109404No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA97670.26645489227223523No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA89760.24487551070293676No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG87370.2383553182945141No Hit
TAGCTTATCAGACTGATGTTGACTCGCCTTGGCCG85820.23412674162796382No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG83640.2281794531550093No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA80770.22034976603694523No Hit
CCCAGTGTTCAGACTACCTGTTCCGCCTTGGCCGT78770.21489353808010614No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA78180.21328395083283863No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA75330.20550882599434298No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG73460.2004072528546985No Hit
AGCTTATCAGACTGATGTTGACCGCCTTGGCCGTA72210.19699711038167403No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA71330.19459637008066488No Hit
TCCCTGAGACCCTAACTTGTGAACGCCTTGGCCGT70690.19285037713447636No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGAGTT69880.19064060481195658No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA68810.18772152285504767No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC66230.1806829887907253No Hit
TAGCTTATCAGACTGATGTTGAACGCCTTGGCCGT63840.17416279638230261No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT60080.1639050878234452No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA59760.16303209135035093No Hit
TAGCTTATCAGACTGATGTTGCGCCTTGCGGCATG58700.16014029053322626No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA57610.15716664629674895No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTCTAT57090.1557480270279708No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT57030.15558434018926565No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG56860.1551205608129343No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC56840.15506599853336592No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT54390.14838211928623807No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC53690.14647243950134442No Hit
AACCCGTAGATCCGAACTTGTGACGCCTTGGCCGT51410.1402523396305479No Hit
CCCAGTGTTCAGACTACCTGTTCGCCTTGGCCGTA50380.13744238223277577No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA48670.1327773073296784No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG48490.13228624681356288No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC48010.13097675210392148No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA47310.12906707231902784No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC44500.12140107203966895No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC44470.12131922862031637No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC44350.12099185494290604No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA44180.12052807556657472No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC43940.11987332821175403No Hit
ACAGCAGGCACAGACAGGCAGACGCCTTGGCCGTA43750.11935498655585432No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT43110.11760899360966583No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC43040.11741802563117645No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC41690.1137350717603101No Hit
TCTCACACAGAAATCGCACCCGTCCGCCTTGGCCG41680.11370779062052588No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC41410.11297119984635264No Hit
TCGTACCGTGAGTAATAATGCGCGCCTTGGCCGTA41280.11261654502915809No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAC40640.11087055208296957No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA40590.11073414638404862No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG39960.10901543457764432No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC38730.10565985438418829No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC38510.105059669308936No Hit
AACCCGTAGATCCGATCTTGTCGCCTTGGCCGTAC37580.10252252330900584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)