FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743885
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1579655.756983255493579No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT867623.162012985238084No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA801252.920129670157459No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA545181.9868908500174025No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA476041.734912359665219No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT332681.212441483516984No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA281611.0263185228243896No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT264370.9634879012786615No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA257850.9397259724806252No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA206910.7540767925769484No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAT184990.6741900626301758No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC180350.6572797329334137No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC168660.6146759066068731No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC155980.5684640573493422No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT125410.4570526826014939No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC116410.42425247413794676No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA115340.4203528937983917No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA113600.4140115201621059No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA112060.40839904004723226No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT111700.40708703170869043No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA99920.3641552032975143No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC95680.3487026606435765No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA89850.3274554144944121No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA85010.3098161912762379No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC80490.2933431976923231No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA79390.28933428332455624No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC77010.2806604504197516No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG75680.2758133085023607No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGATCG72450.2640416781315544No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT71620.2610167700176939No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA71500.26057943390484656No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT69930.25485761976176113No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC68930.25121315215470036No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA68910.25114026280255913No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT64760.23601572223325684No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC63400.2310592462876542No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC63190.23029390809017142No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT61550.2243169812145917No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC59910.21834005433901202No Hit
TGACCTATGAATGTCACTAACATAAGGTTAATGCA59760.2177933841979529No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC58040.21152489991380835No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC57400.20919244064528947No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA55110.20084660982512023No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC54280.1978217017112598No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT53440.1947603489213287No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCGT52940.19293811511779832No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA49990.18218693567696898No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47840.17435133032178826No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT47620.1735495474482349No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAAGC47530.17322154536359943No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA46190.16833795877013796No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA45360.1653130506562775No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA45170.16462060181093596No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAA44580.1624703659227701No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG43740.15940901313283903No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA42980.15663921775147283No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT42110.15346853093332993No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG39280.1431546876053479No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT38930.1418791239428766No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA38270.13947377532221647No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37320.13601153109550876No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC37110.13524619289802597No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT36870.13437152067233138No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC36810.13415285261590773No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA36710.13378840585520166No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35230.1283945937967517No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA35030.12766570027533952No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA34980.12748347689498649No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT34820.12690036207785677No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA34760.12668169402143312No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG33870.12343811785114903No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA33860.1234016731750784No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT33850.1233652284990078No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT33480.12201677548439531No Hit
TACCCTGTAGATCCGATAAACAGCGGAACCGGCAT32150.11716963356700445No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA31490.11476428494634433No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA29760.10845935598612914No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA29720.10831357728184672No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG29660.10809490922542309No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29190.10638200945010451No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA29110.10609045204153965No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC28620.10430466291407985No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA28080.10233665040626702No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC27870.10157131220878425No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC27810.10135264415236062No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT27530.10033219322238358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)