FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581218
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2525417.051818682917377No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA873572.4393097543908246No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA571931.5970264865193908No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT477911.3344901092309935No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT365371.0202394827681531No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA361591.0096844146321167No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA267670.7474272719504929No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT264310.738044989162905No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC262950.734247398510786No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA255000.7120482472722968No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC217300.6067768005187062No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA214190.5980926042480519No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA203700.568800893997517No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA181640.5072017397432941No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA180380.5036833836979486No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC174870.48829755686473153No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT161410.4507125787930251No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC149970.41876813977814253No Hit
CGCGACCTCAGATCAGACGTGCGCCTTGGCCGTAC133760.3735042100201663No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC130330.363926463007837No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT129920.36278160112006586No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA125710.35102582417490363No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT113070.3157305698787396No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA112600.31441816722690435No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC111510.31137451001307376No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT107580.3004005899668772No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA106390.29707769814627316No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT98360.27465515922236516No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA97190.27138811432311577No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT91430.2553042009729651No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA87880.24539137243250758No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC87270.24368804133118957No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA84150.23497592159985792No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG83840.2341102943188602No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80400.22450462384585357No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA78080.21802638096870952No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA77840.21735621791245324No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA75660.21126890348479205No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA75400.21054289350718106No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT74720.20864409818112162No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC74500.20802978204622005No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT72740.2031152529670073No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC71140.19864749925863212No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC70560.1970279385393461No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA66230.18493708006605572No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC64850.18108364249258213No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63950.1785705310316211No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT62640.17491255768288888No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA59320.16564196873801038No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA57330.16008520006321872No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC56000.15637137979313182No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGGTGT55200.15413750293894424No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA55010.15360695718607467No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC54660.1526296360623676No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA53420.14916712693837683No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52980.14793849466857364No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC52880.1476592600618002No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC50930.14221418522971793No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC50760.14173948639820305No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT50580.14123686410601086No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA48590.1356800954312192No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA48290.13484239161089887No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46540.1299557859923635No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA46190.1289784648686564No Hit
CTGACCTATGAATTGACGATTATTCCACGGCATGT45650.12747059799207977No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA45410.12680043493582352No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA44360.12386847156470229No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC43450.12132743664306389No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA43430.12127158972170922No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG43360.12107612549696778No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC42790.11948448823835914No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC42400.11839547327194266No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC40560.1132575565073112No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGACAC39190.10943204239451494No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT38270.10686308401219921No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC37830.10563445174239602No Hit
CTGACCTATGAATTGACAGCCGCCTTGTAATGCAC37120.10365188603430453No Hit
CAACGGAATCCCAAAAGCAGCTCGCGAACCGGCAT36140.10091538688792473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)