FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065647
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT431802.0903862082921236No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA307321.487766302761314No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA213461.033380824506801No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN202310.9794025794339498No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA198860.962700790599749No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN177990.8616670708983674No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT144450.6992966368406606No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCATG143230.6933904970210302No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC126730.613512376509636No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125150.6058634413333933No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121580.5885807207136553No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC121430.5878545559817335No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA95020.4600011521813746No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC93750.4538529574511037No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA91310.442040677811843No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC87720.42466113522784865No Hit
GTCCAGTTTTCCCAGGAATCCCTCGGGAACCGGCA87610.4241286144244394No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAGCAAC84920.4111060602319757No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA76600.3708281231013818No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC74240.35940313131914603No Hit
CTGACCTATGAATTGACAGCCACGCCAACCGGCAT73810.35732145908763696No Hit
TCCCTGAGACCCTTTAACCTGTCGCCAACCGGCAT63400.3069256266922664No Hit
GTCCAGTTTTCCCAGGAATCCCCGAAGGTTAATGC56120.2716824317029967No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC55840.2703269242034094No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACCA55100.2667445115259287No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA54700.264808072240804No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC54480.26374303063398535No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC54340.26306527688419173No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA54280.262774810991423No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACC53360.2583210006356362No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGGT48090.23280841305411815No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT47890.2318401934115558No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA47820.23150131653665895No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCTACG47290.22893553448386875No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA45980.2225936958250853No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT45640.22094772243272928No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA44660.21620344618417378No Hit
TAGCTTATCAGACTGATGTTGACCGTTGGTTAATG40260.194902614047802No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT39890.1931114077090616No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA39190.18972263896009336No Hit
TAGCACCATCTGAAATCGGTTACGTTCCAATTACG38850.1880766655677374No Hit
TAGCTTATCAGACTGATGTTGACCGTCAACCGGCA38300.1854140615506909No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA37840.18318715637279748No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG37650.18226734771236325No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA37280.1804761413736229No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA35360.17118123280502429No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCTAC34140.16527509298539392No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA30340.1468789197767092No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCTACGT30050.14547500129499377No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA29960.1450393024558407No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAA29760.14407108281327835No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA29200.14136006781410376No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC28350.13724513433321378No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC28340.13719672335108565No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG27420.1327429129952988No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA27240.13187151531699268No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC27010.130758062728046No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA26780.12964461013909928No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA26660.12906367835356186No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC26520.12838592460376821No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG26250.12707882808630902No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT25360.12277025067690656No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG25040.1212210992488068No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC24580.11899419407091338No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA24400.11812279639260725No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA24080.11657364496450749No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC23380.11318487621553924No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCTACG22940.11105479300190207No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC21310.10316380291501889No Hit
CAACGGAATCCCAAAAGCAGCTCGAAGGTTAATGC20950.10142100755840663No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC20940.10137259657627853No Hit
TAGCTTATCAGACTGATGTTGACCGTCAACCGATG20890.10113054166563794No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC20850.10093689773712546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)