FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267586
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1049993.2133507733231816No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA1009403.089130630379736No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT647211.9806976771231117No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA645271.9747605724837847No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT566291.7330530856724198No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA532441.629459790805812No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN462421.415173158411133No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA386671.183350644787926No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA334671.0242117575482328No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT296690.9079791625989339No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA264030.8080276999595419No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA241990.7405772946756413No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT241230.7382514186313688No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC217950.6670061629594447No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197070.6031057790062756No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN194980.5967096198845264No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA182400.5582102506253852No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC170430.5215777029280944No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA160610.49152493614552145No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA154380.4724588733089198No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT148060.4531173777828648No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG147570.45161779980695227No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT141180.432062078855767No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN131640.40286621377371556No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC131320.40188689754454815No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA114070.34909563206599614No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC92360.2826551466434242No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC89930.27521846402818473No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA85430.26144682955551896No Hit
AAACCGTTACCATTACTGAGTTTCGCCTTGGCCGT82730.2531838488719195No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA80930.24767519508285318No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA79950.24467603913102826No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA79150.24222774855810988No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC74170.2269871397416931No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA72290.22123365689533497No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA69030.21125687281069266No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT67810.20752322968699216No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT67770.20740081515834624No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA65400.20014775433607562No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG60780.18600887627747212No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT59980.18356058570455375No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC58860.18013297890246807No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA58710.17967392442004587No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA58480.17897004088033183No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA57970.17740925564009638No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT57860.1770726156863201No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC56380.17254327812642115No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC55370.16945231127811172No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT54300.16617772263683345No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA52690.16125053785883525No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA51790.15849621096430208No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA51690.15819017464268728No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC51080.15632335308083703No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG49330.15096771745257814No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT44500.13618616311858356No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA44280.13551288321103103No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT43170.1321158800411068No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC42590.130340869375741No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT42480.1300042294219647No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC42120.12890249866415146No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC41760.1278007679063382No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC41330.12648481172339457No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC40970.12538308096558132No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT40800.12486281921883617No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT39220.12002744533732243No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG38310.11724251481062778No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG38170.11681406396036709No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG38070.11650802763875229No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG36340.11121359927481633No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT35640.10907134502351278No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG34930.10689848714004772No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC34900.1068066762435633No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA34530.10567434185358857No Hit
AGGCAAGATGCTGGCATAGCTCGCCTTGGCTACGT33880.10368510576309238No Hit
CGCCTTGGCCGTACAGCAGACCACTCCCTAGGAGA33840.10356269123444647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)