FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986294
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3377005.641219759671008No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1985063.3160082014014014No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1284182.145200352672288No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1243032.076459993445026No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN970021.6204015372449132No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT831721.3893737928675072No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA818321.3669893259502457No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN797361.331976010533395No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA589250.9843318754474806No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA551630.9214883198185722No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT535880.8951782187777613No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN423220.7069816484121896No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC422290.7054280995888275No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN422080.7050772982416166No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA388260.6485815765146182No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA327340.5468157761713675No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA317570.5304951611130359No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC314580.5255004181217963No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT308330.5150599018357601No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA272210.4547220701155005No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCAATTAC234500.3917281710520733No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA222340.3714151025659615No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT219460.36660411266135606No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA213940.357383048677529No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA213540.35671485563522276No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC204790.34209813283477225No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC196830.3288010912928767No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA195640.32681321699201543No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA184290.30785323941657394No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA177940.2972456748699613No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA161870.27040101939530536No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC151440.25297788581716835No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT150470.25135751768957554No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA141440.23627305975951066No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT132990.22215748174078986No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA123640.20653846937687992No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC123230.20585357150851596No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT121260.20256272077515736No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA119390.19943891830237537No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG116080.1939096208772907No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA115110.19228925274969788No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC114530.19132037283835376No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAG113020.18879794410364745No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA112260.18752837732326544No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC110240.18415400245961858No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA109150.1823331764193339No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT102330.17094048504801135No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC100270.16749929088013385No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC96000.160366330153514No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA95690.15984848054572662No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA95440.15943085989428518No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG93300.15585602711794644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC90630.1513958385605518No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT90440.15107844686545632No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT87590.14631757143902388No Hit
TCAATCCCCGGCACCTCCACCACGCCTTGGCCGTA87120.14553244461431397No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA85940.14356127513951036No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA85590.14297660622749234No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA84510.14117248501326532No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC84470.14110566570903468No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA84450.14107225605691937No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC84330.14087179814422746No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA78700.13146698107376617No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT77230.1290113716432905No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG76900.12846011238338778No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT76790.12827635929675354No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC74730.12483516512887605No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC74040.12368253213089768No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA73120.12214568813359317No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG72820.12164454335186345No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC72820.12164454335186345No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC66400.11092004502284719No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT66120.11045230989323276No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA65740.10981752650304179No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC65560.10951683963400394No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG62400.1042381145997841No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC62000.10356992155747781No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA61980.10353651190536249No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG61800.10323582503632464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)