FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091306
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1556625.035476914934982No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA945523.0586425284329666No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT757832.451488141258096No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA516871.672011764606933No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT511081.6532818168114058No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA466611.5094267600813378No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN404881.3097376966240157No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA376911.2192581387931185No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA341621.1050992687233163No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT336141.0873721333313493No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA314811.0183721702089668No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN256110.8284847892767653No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA248860.8050319185483417No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA228270.7384257656796189No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT207750.6720460543213774No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC207310.6706227076840662No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA175160.5666213567987123No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA168300.5444300887715419No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA161830.5235004234456245No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA160390.5188421980871516No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157370.5090728643492427No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC155110.5017620384394169No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGAC144980.46899271699404715No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA143520.4642697940611508No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA130560.4223457658348931No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA119570.38679444868932417No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA117770.3809716669912328No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113140.3659941785122534No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC112380.36353567068417036No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT99680.3224527109254147No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA98900.31992950552290844No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT97990.31698576588665117No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT97130.31420377018645196No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA85670.27713206004193697No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT78030.2524175866122603No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGCA75290.24355401891627682No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT75180.243198182256949No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC73040.2362755417936626No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA70610.22841478650123928No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC67700.21900128942265823No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC67230.21748089642371218No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA66350.21463420314908974No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG64700.20929665325917268No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64540.20877907266378676No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA64420.20839088721724733No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT64350.20816444570676598No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC63880.20664405270781994No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC63480.20535010121935518No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA56940.18419399438295658No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC56770.18364406500035907No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA56200.1818001841292968No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA55930.18092676687458312No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA55420.17927697872679058No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54840.17740074906851666No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC53590.17335715066706434No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT53300.1724190358379274No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA52990.17141622343436722No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC52920.17118978192388587No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA52630.17025166709474895No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC50410.1630702363337696No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA50370.16294084118492314No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC50070.16197037756857458No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA47900.15495069074365334No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA47450.1534949953191305No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46240.14958079206652464No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA45360.1467340987919022No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA44540.14408149824054947No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT42830.13854985562736266No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC41410.13395632784331282No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC39850.12890991703830032No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT39300.12713073374166128No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG39280.12706603616723805No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC39210.12683959465675673No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA38890.12580443346598494No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA37680.12189023021337907No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGTG37460.12117855689472345No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG36490.11804072453519646No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA36210.11713495849327113No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA34950.11305901130460717No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT32700.10578053418199297No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC31930.10328967756669835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)