FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332220
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT32243313.82515371620173No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA999544.285787790174169No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGT497492.133117801922632No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN385781.6541321144660452No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGAC354321.5192391798372367No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC341801.4655564226359434No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCA325251.394593991990464No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT305771.3110684240766308No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278781.1953417773623414No Hit
CCACTACGCCTTGGCCGTACAGCAGTCTTCGCTAC277911.191611426023274No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN257431.1037980979495932No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGTG233160.9997341588700895No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA224650.9632453199097856No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGC219630.9417207639073502No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA181540.7783999794187513No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA166870.7154985378737855No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG149680.6417919407260035No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT147480.6323588683743386No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT126960.5443740298942639No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGAA122070.5234068827126086No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG109470.46938110469852756No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA105060.45047208239359926No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100760.4320347137062541No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG91460.39215854421967056No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC89910.3855125159719066No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAATG85050.36467400159504676No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG82430.35344006997624583No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA80190.34383548721818696No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT75930.3255696289372358No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA73410.31476447333441954No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGG70500.3022870912692628No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA69660.29868537273499074No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGTT63460.27210125974393495No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCC62200.2666986819425269No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA61160.26223941137628526No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA61130.26211077857148984No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT60420.25906646885799794No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC56150.2407577329754483No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA55400.2375419128555625No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCG54990.23578393119002497No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC53790.2306386189982077No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC52220.22390683554724683No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA47870.20525507885190936No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG46670.20010976666009211No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG45740.19612214971143374No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATAC45090.19333510560753273No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC45000.19294920719314643No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC43950.18844705902530634No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA42740.18325886923189064No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG37420.16044798518150089No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA36200.1552169177864867No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGACAC36040.15453087616091107No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGTCTT35200.150929157626639No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT34880.14955707437548774No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA34720.1488710327499121No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG34610.14839937913232884No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGACTG34510.14797060311634408No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT31820.13643652828635378No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGACGT31820.13643652828635378No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC31260.13403538259683906No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT31160.1336066065808543No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAACA31150.1335637289792558No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGAT29960.12846129438903706No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA28100.12048606049172034No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC28040.12022879488212948No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA27440.11765613878622085No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGCA26640.11422593065834269No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA26240.11251082659440362No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA26140.11208205057841884No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT25450.10912349606812394No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAAGT25140.10779429041857115No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC24720.10599343115143513No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGA24630.10560753273704881No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGAG24430.10474998070507929No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGAGTA23870.10234883501556458No Hit
CACTACGCCTTGGCCGTACAGCAGTCTTCGCTACA23770.10192005899957979No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)