FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783825
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1532064.048971609416398No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1040072.7487264870864796No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA991082.6192543259796635No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN753771.9920847290770582No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA504331.3328576242294503No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN463751.2256116495873886No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA446481.179970003898172No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA417571.103565836157856No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT345750.9137579037085489No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC311880.8242453073279024No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA310170.8197260708410141No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT272580.7203821529801193No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA251970.6659134605855186No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246450.6513250480664406No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA242760.6415730114368398No Hit
TGTAACAGCAACTCCATGTGGACGCCTTGGCCGTA219990.5813958097956433No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA214310.5663845447397805No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211670.5594074778828302No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT206730.5463519058095975No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA206510.5457704835715183No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT202340.5347498893315626No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT201930.5336663297060514No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA180620.47734765746301694No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA178880.4727491361254815No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT176450.46632706322306133No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC167880.44367802422152186No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT155530.4110390940384399No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA145830.3854036589958574No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT142610.3768937516930619No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT136280.3601646482065106No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC136230.36003250678876536No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA132770.35088832068079256No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA114270.3019959961150423No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA107820.2849497532259024No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG107390.28381333703329303No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA104250.27551485599889003No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT103610.27382344585175056No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA96580.2552443625167654No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA93980.24837300879401136No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT87970.2324896103810298No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT86640.22897464866900555No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT83690.2211783050220346No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA82970.21927546860650268No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC82140.2170819210719312No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA81910.21647407055030293No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG78490.2074355975765264No Hit
TGTAACAGCAACTCCATGTGGCGCCTTGGCCGTAC76190.20135709236024393No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT73980.19551644169590296No Hit
ACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGTA73340.1938250315487635No Hit
CAAAGTGCTTACAGTGCAGGTCGCCTTGGCCGTAC72190.19078577894062224No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA71470.18888294252509036No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA69900.18473370200788886No Hit
CCACTACGCCTTGGCCGTACAGCAGAGCTTACCGC69640.18404656663561342No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA69610.18396728178496627No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA65140.17215383903853904No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG64770.17117599254722404No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC64490.17043600060785052No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA63840.16871816217716198No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA61590.16277179837862482No Hit
CAAGTCACTAGTGGTTCCGTTTCGCCTTGGCCGTA60420.15967968920338546No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA59600.157512569952363No Hit
CGCCTTGGCCGTACAGCAGAGCTTACCGCAGAGAC57500.15196263040706165No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC56750.14998050914088257No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC56740.14995408085733353No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC56060.14815695757599784No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC55420.14646554742885837No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT55390.1463862625782112No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGAT52740.13938276743771183No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA51680.1365813693815121No Hit
TAGCTTATCAGACTGATGTTGCGCCTTGCGGCATG51330.1356563794572952No Hit
TAACACTGTCTGGTAACGATGTTCGCCTTGGCCGT49400.13055572073232774No Hit
CAAAGTGCTTACAGTGCAGGTACGCCTTGGCCGTA48310.1276750378254808No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA46990.12418650439700568No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA46740.12352579730827931No Hit
CGCCTTGGCCGTACAGCAGAGCTTACCGCAGATAC46400.12262723566761147No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC44570.11779085977813455No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAA44450.1174737203755459No Hit
CAAAGTGCTTACAGTGCAGGTATCGCCTTGGCCGT43720.11554445567646496No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT43640.11533302940807252No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC43530.11504231828903293No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGAGCT43210.11419661321546319No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT42890.11335090814189346No Hit
AGCTTATCAGACTGATGTTGACCGCCTTGGCCGTA42840.11321876672414818No Hit
AATGACACGATCACTCCCGTTGACGCCTTGGCCGT42610.11261091620251994No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA42430.11213520709863697No Hit
CAAAGTGCTGTTCGTGCAGGTAGCGTAGGTTAATG42180.11147450000991062No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA42070.11118378889087102No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA41340.10925452419179005No Hit
TAAAGTGCTTATAGTGCAGGTAGCGCCTTGGCCGT41240.1089902413562995No Hit
TCAGTGCACTACAGAACTTTGTCCGCCTTGGCCGT40430.10684955038882613No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA40310.10653241098623747No Hit
CGCCTTGGCCGTACAGCAGAGCTTACCGCAGAGCA39260.10375744121358678No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC39110.10336101696035098No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG39070.10325530382615475No Hit
TGTAACAGCAACTCCATGTGGAACGCCTTGGCCGT38860.10270030987162462No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC38840.10264745330452651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)