FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617011
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN446271.7052660458821152No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN368281.407254306535204No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGATGCA240300.9182231179005361No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT237310.906797869783505No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179720.6867376560511209No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT166380.6357634721443662No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATCGTAC153550.5867380763779747No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148770.5684729640035904No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC60590.2315236733815792No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGCGGCA56080.21429027237562243No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGAGCAT55990.21394636858614655No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT53520.2045081201416425No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCTCGTA52410.2002666400714403No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA51210.1956812562117622No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA47670.18215437382571184No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA45910.17542914416485067No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC45150.17252506772038786No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGCGGCA40580.15506239752144718No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGGGCA39250.1499802637436373No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCTACG38870.14852822552140593No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGTCGTAC38820.14833716786058598No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA37040.14153551513539683No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGCGGCA36380.1390135540125739No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGCGATGC35940.1373322465973586No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT35320.13496313160319157No Hit
CTGACCTATGAATTGACAGCCCGCCTTATAGCATG33630.1285053826674783No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGCGATGC33040.12625090226980323No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCTACG32680.1248752871118998No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCTACG32470.12407284493645614No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCTACG31570.12063380704169759No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGGGCAT31560.12059559550953358No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGATGC30960.11830290357969454No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCTTACG30600.11692728842179112No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGATGCAC30470.11643053850365934No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGGATGCA29860.1140996350416563No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCCTACGT29740.11364109665568849No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCCGTA29340.11211263536912913No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT29210.11161588545099733No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGATGC29180.11150125085450538No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGCGGCA29040.11096628940420962No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT28890.11039311642174986No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCAGCATG28750.10985815497145407No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA28410.10855896287787863No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG28240.1079093668310909No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT28040.10714513618781121No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG27370.10458496353282429No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCAATG27280.10424105974334844No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC27250.10412642514685648No Hit
CTGACCTATGAATTGACAGCCGCCTTGCTCGTACA26950.10298007918193697No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA26750.10221584853865727No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA26420.1009548679772458No Hit
CGCCTTGGCCGTACAGCAGCACTTACGGCAGAATG26200.10011421426963814No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)