FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573180
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1310743.6682730788821165No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN646021.8079693718200593No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA590441.6524216524216526No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA584661.6362455851650353No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT466551.3056996848745375No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN432371.2100425951113574No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA346190.968856872589682No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN270480.7569727805484191No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA267240.7479052272765436No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC265250.7423359584459781No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA258980.7247885636883672No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT238130.6664371792073167No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT233390.6531716846058693No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAGAC230590.6453355274573349No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA224730.6289355699964737No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN202550.5668620108698694No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC199570.5585221007617864No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT173800.4864014687197399No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC157670.4412596062890758No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT147770.41355319351390074No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA139270.3897648593129929No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC138470.3875259572705545No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA117980.33018207870860133No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT110530.3093323034383938No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT100120.28019859061116426No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT98470.2755808551486351No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA94080.2632948801907545No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC90360.25288398569341597No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC80660.22573729842885049No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA78630.2200560844961631No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA78330.21921649623024866No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC77390.21658578633038358No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA76710.21468271959431096No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA71370.1997380484610347No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA71330.19962610335891282No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA67130.18787186763611124No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT66830.18703227937019684No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC66460.1859967871755691No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAAGT66040.18482136360328896No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA65130.18227461253001528No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA61500.1721155945124511No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA60990.17068829446039663No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT59940.16774973552969624No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA58160.1627681784852708No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG57000.15952177052373517No Hit
CTGACCTATGAATTGACAGCCCACCTTGGCCGTAC55570.15551973312287654No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA54250.1518255447528532No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA53600.150006436843372No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA52420.14670405633077538No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA51220.1433457032671178No Hit
CTGACCTATGAATTGACAGCCCGACTTGGCCGTAC49290.13794435208973518No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA47430.13273890484106593No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA46800.13097576948264572No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA46330.12966041453271315No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA45920.1285129772359635No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT45480.1272815811126224No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG43930.122943708405398No Hit
CATTGCACTTGTCTCGGTCTGACGCCTTGGCCGTA43910.12288773585433703No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA42970.12025702595447194No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC42610.11924952003537466No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA41590.11639491993126572No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT40710.1139321276845835No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC40110.11225295115275469No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT39170.10962224125288958No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT38660.1081949412008351No Hit
CTGACCTATGAATTGACAGTTTATTCCAATTACGT38210.10693555880196351No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA38020.1064038195668844No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA37600.10522839599460423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)