FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692370
Sequences flagged as poor quality0
Sequence length35
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC33792312.551135245155756No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA853393.1696609307041754No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN595162.2105431274304794No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT397121.4749830075361114No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA356531.324223639395774No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT327281.2155832965008524No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC262170.9737517503166355No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249900.9281785192971249No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT231800.8609515036937717No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC202500.7521254508109955No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA190680.7082236096821759No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA178980.6647674725242073No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172880.6421108540059501No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN164870.6123601139516486No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT154570.5741038564536078No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA151790.5637783811288938No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA145990.5422360225377642No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA145430.540156070673793No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA143510.5330248071401776No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT136430.5067282728599709No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA134790.5006369852583412No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG120530.44767249672221876No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC120280.4467439467829458No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC100500.37327707558767925No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGAT99020.3677800599471841No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACCTA83700.3108785196685448No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT75870.28179633557051964No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA68820.2556112272830257No Hit
CGCCTTGGCCGTACAGCAGAAGTTTGCTGAGAGTC66560.2472171358319993No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT62830.23336317073804863No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGCGT61560.22864613704654263No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGCG60110.22326054739876022No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACT57370.21308364006432995No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA52660.19558975920842978No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC52330.1943640732885896No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT49670.18448430193472665No Hit
CGCCTTGGCCGTACCTACTCCTGGGTAGTCTCTGA48760.18110438015577354No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA48400.17976726824322067No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA45740.1698874968893577No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA42220.1568135137443962No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA40390.15001652818891906No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC40130.14905083625207532No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGGC36920.13712825503181214No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC36850.13686826104881572No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC34970.12988556550548402No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGCGT34550.12832560160750567No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT32720.12152861605202851No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACT32480.12063720811032659No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT32320.12004293614919198No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA31750.1179258422876499No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA30430.11302309860828935No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC30160.11202026467387469No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA29330.10893747887548888No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA28520.10592897707224488No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA28180.10466614915483384No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACCTA28090.10433187117669562No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA27890.10358903122527736No Hit
CGCCTTGGCCGTACAGCAGAATGGGTAGTCTCTGA27630.1026233392884336No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT27470.102029067327299No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA27260.10124908537830983No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA27170.10091480740017159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)