FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281055
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN443931.9461608773133483No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN252001.1047519678394428No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN160500.7036217890405975No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152030.6664898478993273No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC91110.39942044361052237No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGCCGGCAT82550.3618939481950238No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG71990.31559958001889477No Hit
TAGCACCATCTGAAATCGGTTACGCCTTAATTACG64940.28469282853767225No Hit
CGCGACCTCAGATCAGACGTCGCCTCGCGGCATGT63810.279738980427916No Hit
CTGACCTATGAATTGACAGCCCGCCTTATAATGCA62020.27189173430715174No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG60160.2637376126397654No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTAATTACG55760.24444829256637826No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTAATTAC53680.23532970489532254No Hit
AAACCGTTACCATTACTGAGTTCGCCTTATAATGC52220.22892915778006231No Hit
TAGCACCATCTGAAATCGGTTCGCCTTATAATGCA52040.22814004923160552No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG51220.22454522139974706No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATTACGT47580.20858769297539956No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG47470.20810545997356483No Hit
CTGACCTATGAATTGACAGCCGCCTTATAATGCAC46090.20205562776872982No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT45840.200959643673651No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTCATAATG43380.19017516017807548No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT41990.18408148860943732No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTATAATG41750.18302934387816164No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT40940.1794783554101063No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGCCGT40810.17890844368066533No Hit
CTGACCTATGAATTGACAGCCCGCCTTAGCCGTAC39440.1729024508396334No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTATAATG38250.16768556654705827No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTACGGCA35680.1564188500496481No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGCGGCA34880.15291170094539588No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG34410.15085125084664772No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCGCGGCA33010.14471373991420636No Hit
TAGCACCATCTGAAATCGGTTACGCCTTATAATGC31590.1384885502541587No Hit
ATCACATTGCCAGGGATTTCCACGCCTTAATTACG31330.13734872679527674No Hit
TAGCACCATCTGAAATCGGTTCGCCTCGCGGCATG31240.13695417252104838No Hit
CGCGACCTCAGATCAGACGTCGCCTCGCGGCTACA31150.13655961824682No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTCAATTAC31000.13590202778977273No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTATAATGC30650.13436765005666237No Hit
TAGCTTATCAGACTGATGTTGACCGTCTTAGCCGT30440.13344702341679618No Hit
TAGCACCATCTGAAATCGGTTACGCCTGCCGGCAT30330.1329647904149615No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA29990.13147425204565433No Hit
TAGCTTATCAGACTGATGTTGACCGTCTTACGGCA29470.12919460512789038No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTAGCCGT29380.128800050853662No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC29370.12875621148985886No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA28880.12660808266350437No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC28610.12542441984081928No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTAATTACG28500.12494218683898459No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC28190.12358316656108687No Hit
AAACCGTTACCATTACTGAGTTCGCCTTAATTACG27920.12239950373840174No Hit
TAGCACCATCTGAAATCGGTTACGCCTGCGCCGTA27240.11941842699978737No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTCAATTAC27050.11858547908752748No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAATTACG26920.11801556735808649No Hit
TAGCTTATCAGACTGATGTTGACCGTAGGCTAATG25980.11389466716059016No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG25930.11367547034157441No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC25850.11332475543114919No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC25620.11231645006367667No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCGTAATG25440.11152734151521992No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCGTAATG25010.10964224887168438No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTATAAT24650.10806403177477089No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCGATTAC24380.10688036895208576No Hit
CGCGACCTCAGATCAGACGTCGCCTCGCGGCGCAC24070.10552134867418805No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGCGGCA23980.10512679439995966No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG23770.10420616776009348No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTAATTACG23540.10319786239262095No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCGATTAC23210.10175116338711693No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT23120.10135660911288855No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTCGCGGCA22830.10008526756259714No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)