FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225044
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT738162.2888369895108407No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA733452.274232537602588No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN543411.684969259334136No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT383131.1879837918490415No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC345451.071148176583017No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA317850.9855679488403879No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA314170.9741572518080375No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA311770.9667154928738956No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN308440.9563900523527741No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278820.8645463441739089No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN269710.8362986675530629No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA227000.7038663658542333No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA218230.6766729384157238No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT203330.630472018366261No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA176870.548426626117349No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA174390.5407368085520694No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT165180.5121790586423006No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC154940.4804275538566296No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC154690.4796523706343231No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGATTAC122730.38055294749466984No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA120980.37512666493852487No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT110930.3439642994018066No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA109940.34089457384147315No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT98680.30598032150879184No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA93240.289112334591404No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC91710.2843682132708887No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT87140.2701978639671273No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC86630.26861649019362216No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC86280.2675312336823932No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGGCGT84830.2630351709930159No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA82240.25500427280992133No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT82150.25472520684989103No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT80430.24939194628042283No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC80360.24917489497817705No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA77840.2413610480973283No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA75870.23525260430555367No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG73610.22824494797590356No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT66650.20666384706689275No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA65770.2039352021243741No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGTAATG64140.1988810075149362No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG61870.1918423438563939No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA60940.188958662269414No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT58150.1803076175084743No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA57490.17826113380158534No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA56900.17643170139694217No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT56860.17630767208137316No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA56530.1752844302279287No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA56470.17509838625457513No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA54830.170013184316245No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC53950.16728453937372637No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGCGGCA53740.166633385466989No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA52700.16340862326219427No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG52380.16241638873764203No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC50640.1570211135103893No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG48710.1510366990341837No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA48410.150106479167416No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG48400.15007547183852377No Hit
CGCCTTGGCCGTACAGCAGGCCTTACCGCAGATAC48150.14930028861621733No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA48110.14917625930064832No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47870.14843208340723413No Hit
TCCCTGAGACCCTAACTTGTGCGCCTTGGCCGTAC46740.1449282552424091No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT46470.14409105736231817No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC46020.14269572756216659No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT41060.12731609243160713No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA40360.12514557940914914No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA39760.12328513967561373No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC39730.12319211768893697No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT38490.11934720890629709No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAGCCGT36430.11295969915449215No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA35610.11041709818532708No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC35170.10905277571406778No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA33380.10350246384235379No Hit
AACCCGTAGATCCGAACTTGTGCGCCTTGGCCGTA33210.10297533925118542No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC32480.10071180424205066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)