FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4607791
Sequences flagged as poor quality0
Sequence length35
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT65663814.250602946183974No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA3451697.490986461842562No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1836983.9866825556975134No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC930252.018863268755028No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA789901.7142704606176797No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN746271.6195830062604837No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN533961.1588199204347593No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT515351.118431803873049No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT487911.0588804917584154No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN450680.9780825562617749No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA435290.9446826038767817No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA351710.7632941685072088No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA338850.7353849165467792No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA335600.7283316452504031No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA331310.7190213271391867No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG309310.6712761060560256No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN307030.6663279649619525No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA300190.6514835416797333No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA276040.5990723103543542No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT275910.5987901795024991No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA259030.5621565735077828No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA257550.5589446222712793No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC209270.4541655643669602No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC172700.3747999855028147No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG158830.3446987938472036No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA158020.3429409016164145No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA147770.32069596906630526No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT134640.292200753028946No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA134560.29202713404318903No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT119230.2587573958975136No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG117270.2545037307464683No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC110390.23957249797137065No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT107310.2328881670197281No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA105340.22861279949546323No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC105060.22800513304531392No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC103630.22490169367490845No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT101750.22082164750962013No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA97960.21259644805938466No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG96630.20971003242117536No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT96220.20882023511917097No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC94700.20552147438978896No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT94290.20463167708778457No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA94210.20445805810202763No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC90240.19584221593383902No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA89100.19336814538680247No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC85820.18624976697076753No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC84100.18251695877699314No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA78440.17023341553468896No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG76000.164938036469102No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC73380.1592520146855619No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA71300.15473792105588124No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA70490.15298002882509212No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA69820.15152596981937766No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA69370.15054936302449481No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA68950.14963786334927084No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC68560.1487914707937057No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA67060.1455361148107629No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC65600.14236756832069858No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGATAC65290.1416947947508904No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT64710.14043605710415252No Hit
CCACTACGCCTTGGCCGTACAGCAGTGTCCCTCGC63870.13861305775370455No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC62880.1364645228049623No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGAA62750.13618239195310725No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA61090.13257979799865055No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC60890.13214575053425817No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT60100.1304312630499083No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGAC59970.13014913219805327No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA59890.1299755132122963No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT59670.1294980610014647No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA59340.12878188268521726No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA59300.1286950731923388No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA58890.1278052758903344No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC58310.12654653824359657No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC57360.12448481278773278No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC57080.12387714633758345No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG56900.12348650361963033No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA56510.12264011106406518No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT55850.12120775443157036No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC55310.12003582627771095No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA53360.1158038634998853No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA50970.11061699630039643No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT49260.10690589047984164No Hit
CGCCTTGGCCGTACAGCAGTGTCCCTCGCAGAGCA48350.10493097451685633No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46540.10100284496410536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)