FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3968480
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1064792.6831179696004517No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN803822.025511026891908No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA592461.4929141636092407No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC579451.460130830947869No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT571111.4391152279966133No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA480181.2099846792726685No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA478331.205322944805064No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT474041.194512760553159No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA467241.1773777365641251No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN369960.932246099262186No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA360510.9084334556303673No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA315840.7958714671612305No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN306770.7730163689876225No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC290220.7313127444260775No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286750.7225688424787324No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA285270.7188394549046486No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA254640.6416562512599282No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT250660.6316272225134056No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA224110.5647250332621054No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT220490.555603152844414No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGAC219400.5528565092932307No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC219360.5527557150344716No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT212350.5350915211869531No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC185810.46821453050034273No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT165770.4177166068620731No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA163990.4132312623472967No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA162550.40960266903197196No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA157750.39750735798088943No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC152890.38526085554166833No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC149640.3770713220174979No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT144290.3635900899084788No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA140670.3544682094907874No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA127720.32183606821755434No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA127390.32100451558279236No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGCA118730.2991825585614643No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA114780.289229125509011No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT113630.28633129056968915No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA113460.28590291496996334No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA112410.283257065677539No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA107860.27179171874370034No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA102380.2579829052937145No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT101920.2568237713179857No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA100430.2530691851792122No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA93800.23636253678990443No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA93330.23517820424948593No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTAC91770.23124722815788412No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC91110.22958412288836028No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC90880.22900455590049593No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC90660.2284501874773213No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG90600.22829899608918275No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA90210.2273162520662823No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA89470.2254515582792404No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC88140.22210014917550297No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC84910.213961012780712No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC83700.21091198645325163No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA79990.20156331895335242No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA76880.19372656533483854No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA75350.18987118493730598No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC74900.188737249526267No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG74740.1883340724912309No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA73510.185234649034391No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC73030.18402511792928275No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT71400.17991775188485262No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA70940.1787586179091239No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT69930.1762135628754586No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT68600.17286215377172115No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC68030.1714258355844051No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA67730.17066987864371247No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG65550.1651765915413458No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT63000.1587509575454582No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG62690.1579698020400758No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG62360.15713824940531387No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG56960.14353102447284605No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT56270.14179232350925292No Hit
TAGCTTATCAGACTGATGTTGACGCGGAACCGGCA55650.1402300124984881No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG53690.13529109381929605No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA53250.13418235697294684No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC53190.1340311655848083No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA50010.12601802201346612No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT49610.12501007942587591No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC49070.12364935693262911No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT48980.12342256985042133No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT48530.12228863443938234No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48330.12178466314558722No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA47210.11896242390033464No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA45990.11588819900818449No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT45710.11518263919687134No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTCGGCA44940.1132423497157602No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT43700.11011772769423053No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA43620.1099161391767125No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA43500.10961375640043544No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC43290.10908458654195057No Hit
ATCCCGGACGAGCCCCCACGCCTTGGCCGTACAGC42950.10822783534249888No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG42580.10729548844897795No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC42520.10714429706083942No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC41510.10459924202717413No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAATTAC40570.10223057694633714No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGTG40550.10218017981695764No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40400.10180220134661129No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA40190.10127303148812644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)