FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074831
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2753906.7583170933960215No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1581233.880479951193068No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT920242.258351327944643No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA561891.3789283531022514No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA525841.2904584263740018No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN479251.1761223962417091No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT479221.1760487735564984No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA479111.1757788237107258No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477591.1720486076600478No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA469391.1519250737024431No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC466461.1447345914468599No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT448881.1015916979133613No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA386410.9482847264095126No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT386370.9481865628292315No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA282100.6922986499317395No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT235160.5771036884719882No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT215720.5293961884554231No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190730.4680684916748694No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA183390.45005547469330626No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182900.4488529708348641No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC170980.4196002239111266No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA164900.40467935970841495No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT151160.370960169881892No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA147100.36099656648337075No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA144200.35387970691299836No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT132430.32499507341531464No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA121620.2984663658443749No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA111030.27247755796498063No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC106480.2613114507080171No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA105970.26005986505943435No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC103300.2535074460756777No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA100140.2457525232334789No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG96610.2370895872736808No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT96020.23564167446453607No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA93610.2297273187526059No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGAT92640.22734685193079174No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT90150.2212361690582996No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA89460.21954284729845236No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC83570.2050882601020754No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT83490.20489193294151342No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC79820.19588542445073184No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT77810.19095270454161167No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC76830.18854769682472722No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC71620.17576189049312718No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC70810.173774077992437No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC67770.1663136458910811No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC67000.1644239969706719No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC65800.16147908956224197No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA64690.15875505020944428No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA63500.15583468369608455No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT63350.15546657027003083No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA62240.15274253091723314No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG61600.15117191363273716No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGCG59550.14614103014333601No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA56630.13897508878282314No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55930.13725722612790567No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC55620.13649645838072794No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC54060.13266807874976902No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG51060.12530581022869416No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG50620.12422601084560317No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC49570.12164921686322697No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA47760.1172073148555118No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA47450.11644654710833408No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC46890.11507225698440009No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC46420.11391883491609835No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA46240.11347709880483386No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA45660.11205372689075939No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC45370.11134204093372216No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC45080.11063035497668491No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC44570.1093787693281022No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA43990.10795539741402771No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA43550.10687559803093673No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA43380.1064584028147425No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT42920.10532952164151103No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT42180.10351349540631256No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT41980.10302267750490757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)