FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704983
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1774524.789549641658275No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA850772.296285839908037No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT671741.8130717468879076No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA380191.0261585545736647No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA373921.009235400000486No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA369100.9962258936140868No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA311440.8405976491660015No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG305300.8240253733957753No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA302970.8177365456197775No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC292900.7905569337295205No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA262570.7086942099329471No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG249820.674281096566435No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT240810.6499624964540998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC239480.6463727363931224No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT222450.6004076132063224No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG217550.5871821814027216No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA204300.5514195341786993No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGTAT195620.5279916264123209No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGTAT192430.5193816004014054No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC175940.47487397378071644No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA139270.37589916067091267No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGAGC127170.3432404413191639No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA123030.3320663009789788No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT117680.31762628870361886No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA116330.31398254728834113No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC110340.297815131675368No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC109460.2954399520861499No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC103610.27965040595327967No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC102350.2762495806323538No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC98470.265777197898074No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT98270.26523738435506994No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC96680.26094586668818726No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA96330.26000119298793006No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT93320.2518769991657182No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGATAA92880.2506894093711091No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT91400.2466947891528787No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA90990.24558817138972028No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC89330.24110771898278616No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88580.23908341819652074No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT84430.22788228717918543No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83010.22404961102385623No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA82850.22361776018945295No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT80610.21757184850780695No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT77970.21044630974015266No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT77740.20982552416569794No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA76060.20529109040446342No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT74050.1998659642972721No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC72360.19530453985888732No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC66480.17943402169456646No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG63460.1712828371952044No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACATA62850.16963640588904186No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA62140.16772006781137727No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG61510.16601965515091432No Hit
GCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61450.1658577110880131No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA59850.1615392027439802No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT59820.1614582307125296No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC59040.15935295789481355No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC56770.1532260741817169No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC56740.15314510215026628No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC55710.1503650624037951No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC55620.15012214630944326No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA55520.1498522395379412No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA54420.1468832650514186No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54120.14607354473691242No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA52880.14272670077028696No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT52540.14180901774717994No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA52310.14118823217272522No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52270.14108026946412439No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG50410.13606000351418618No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT48780.13166052313870266No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGTCG46140.1245349843710484No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA46070.12434604963099695No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45620.12313146915923771No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC45290.12224077681328094No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGATT45200.12199786071892908No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTAA42640.11508824736847646No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC42140.11373871351096619No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC42030.11344181606231392No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA41320.11152547798464932No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGCGG40910.11041886022149089No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG40130.10831358740377486No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT39400.10634326797180986No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA39260.10596539849170698No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAAGGT38200.10310438671378519No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA37910.10232165707642923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)