FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394036
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1928074.3879249054855265No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1887364.295276597642805No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1132992.578472274692333No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA886122.016642558231202No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT590681.3442766513519688No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN486441.1070460050850746No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA483211.0996951322201274No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC461901.0511975778077376No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN408700.9301243776791998No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA404610.9208163064663103No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC401710.9142164515720854No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT392080.8923003816991941No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA387760.8824688737188316No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA316840.7210682843745476No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA311780.7095526754901416No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA308570.7022473188658446No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA305840.6960343520171433No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286360.6517015336242125No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA273550.6225483814879987No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA264660.6023164125191509No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN247290.5627855575147768No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC244220.5557988145750284No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA223820.5093722491122057No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC201060.45757476725270346No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT162020.36872706550424256No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC144930.329833437868966No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT143510.32660178478282836No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA136630.31094419799928813No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG122270.27826353721271285No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC120970.27530498157047417No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA120970.27530498157047417No Hit
CAACGGAATCCCAAAAGCAGCTCGCGAACCGGCAT111420.25357097666018213No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC108290.2464476849984843No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC101730.23151835806534132No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA100060.2277177519710808No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC99070.22546469805891442No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA94990.21617938496634984No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT94390.21481389774685505No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA93950.21381254045255887No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT91500.20823680097295516No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC86640.19717635449504736No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGGTGT85760.19517363990645503No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT84830.1930571347162381No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT80990.18431801651147148No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA80000.18206496259930505No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC78920.17960708560421443No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT77630.17667128808230065No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA75930.17280240762706542No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA75610.17207414777666818No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA75090.17089072551977272No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGT74240.1689562852921551No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT70590.1606495713735618No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA70590.1606495713735618No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG70010.15932960039471686No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT68940.1568944815199511No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC65690.14949809241435438No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA65500.14906568812818102No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC63750.1450830170713212No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC62230.1416237827819344No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC59400.135183234729984No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC58610.13338534322431586No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC57250.1302902388601277No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA56210.1279233943463367No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT55720.12680824645041597No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA54920.12498759682442293No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA54690.12446416005694992No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC53260.12120974885048734No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA53120.12089113516593857No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT52580.11966219666839324No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA52140.11866083937409706No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA52120.11861532313344725No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC51880.11806912824564933No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA51110.11631675298063103No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA50680.11533815380665975No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA50280.11442782899366323No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG50150.11413197342943936No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA49880.11351750418066671No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA49340.11228856568312139No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA48130.1095348331238069No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA47800.10878381515308479No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA47170.10735005357261525No Hit
ACCACAGGGTAGAACCACGGAACGCCTTGGCCGTA45940.10455080477265093No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT45590.10375427056127898No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA44490.10125087732553853No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA44190.10056813371579114No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)