FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5210213
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2120784.070428598600479No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1016301.950592039135444No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA948431.8203286506712872No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN857261.6453454014260067No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT822851.579302036212339No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT712311.3671418039915066No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT633851.2165529509062296No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA510250.9793265649600122No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA508420.9758142325467308No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN429910.8251294140949708No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA411670.7901212484019368No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA350400.6725252883135487No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC333300.6397051329763294No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA331800.6368261719818364No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT306190.5876727112691938No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC286500.54988154994815No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278950.5353907796092022No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA250330.48046020383427707No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA224180.43027031716361697No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA224140.43019354487043043No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC217000.4164896905366441No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT208360.39990687520836476No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT188600.36198136237424455No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC162080.31108133199160953No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT151870.291485204155761No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148860.2857080890934785No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC142350.2732133983773792No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGAGAC139470.2676857932679528No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA135460.2599893708760083No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA117460.22544183894209316No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC117050.22465492293693173No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT112220.21538466853466448No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC111820.21461694560279973No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA111270.21356132657148566No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA105490.20246773020603956No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC103790.19920490774561422No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA100410.19271764897135685No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA98130.18834162825972758No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT96060.18436866208732733No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA95120.1825645131974451No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT92650.17782382409318007No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC91550.1757125860305519No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA91340.17530953149132292No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC91250.17513679383165334No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA89430.17164365449166857No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC86530.1660776632356489No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA84180.16156729101094333No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA81400.15623161663448307No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT80060.15365974481273606No Hit
TAGCTTATCAGACTGATGTTGACACATTCCAATTA77880.14947565483407302No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA76530.14688458993902936No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC73820.14168326707564546No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA73040.14018620735850915No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC72270.13870834071466942No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG72110.13840125154192354No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC70050.1344474784428199No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT69330.1330655771654633No Hit
CGCCTTGGCCGTACAGCAGACGCACCAGCAGTCTG68890.13222108194041204No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT68470.131414972861954No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA67420.12939970016580896No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC66720.1280561850350456No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG66630.12788344737537602No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC66070.12680863527076533No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA63590.12204875309320368No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61560.11815255921398991No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC61350.1177495046747609No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA60300.11573423197861585No Hit
TATTGCACTTGTCCCGGCCCACTATGAACCGGCAT59660.11450587528763219No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA59530.11425636533477614No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC59270.11375734542906403No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG58480.1122410926386311No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA58010.11133901819368996No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC57950.11122385975391026No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT57740.11082080521468124No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT56310.10807619573326464No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT55850.10719331436162016No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC55440.10640639835645875No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC55370.10627204684338241No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG53750.10316276896933005No Hit
TGTGCAAATCCATGCAAACAGTATAAGGTTAATGC53710.10308599667614357No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA53610.10289406594317736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)