FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007139
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2433746.073510302487635No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA968982.418134234924219No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT754571.8830642011669674No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA643101.6048856802821163No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN620981.5496842011220475No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN593381.4808071294756684No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA509921.2725288541275959No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA506351.263619754642901No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA457391.1414378188528025No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA453541.1318299664673475No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC401931.0030348335807668No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA356510.8896871308931384No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT313720.7829027143805093No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN308540.7699757857164427No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA250420.6249346478871833No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC240530.6002536972138975No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA234460.5851057325438423No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217290.5422572064507869No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT207750.5184496969034516No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT201980.5040503960556396No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC190960.4765494783185709No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA186440.46526961006343925No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC184620.46072771620849684No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA166070.41443533653312253No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC157390.39277399660955115No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA153410.3828417232344573No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA137880.34408589270299833No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG132980.3318577169396919No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC128150.31980422940157555No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT125450.3130662550013863No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC117390.29295215364378424No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC113210.28252077105386164No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA108190.2699931297616579No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC102260.25519454154198296No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC101130.25237457447820005No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTT101120.25234961901745856No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA100830.2516259106559568No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC95230.23765085264074942No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC92510.23086296731907727No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA91200.22759380196194842No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC91170.2275189355797241No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA90420.22564727602411597No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA90300.22534781049521865No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT89960.22449932483000964No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT88100.21985760913210148No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA83680.20882729548438425No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA81390.20311249497459408No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA81200.2026383412205067No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA78490.195875411359576No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC75830.1892372588023525No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA73780.18412138935035696No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC73370.18309821545995786No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC72840.18177557604066144No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA70400.17568644361974964No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACAC68290.17042084140330546No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTAATTAC68260.17034597502108112No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG66830.16677734413505496No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA66630.16627823492022614No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT65840.16430675352165225No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA64180.1601641470385729No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA63150.1575937345822044No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA62500.1559716296340107No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA60490.1509555820249809No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA60470.15090567110349803No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC60410.15075593833904938No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG59900.14948320984123586No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT58210.1452657369759322No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA57980.14469176137887904No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA57640.14384327571367003No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC56140.14009995660245378No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC54410.13578266189418436No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54220.13530850814009698No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAATG52780.13171492179332936No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA51630.12884504380806355No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA50050.12490208101091577No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC47600.11878799312926254No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA44820.11185037504314176No Hit
GAAAATTACGAGAACAACATAAGGTTAATGCACTA44070.10997871548753362No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA43750.1091801407438075No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA42580.1062603518370588No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA42440.10591097538667862No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA42150.1051872670251768No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA41340.10316587470512004No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG41260.1029662310191885No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40220.10037086310207856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)