FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002088
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397231.9840786219187168No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT254821.27277122683918No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN203341.0156396721822418No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA194300.9704868117685136No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT169830.8482644119539201No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC161480.8065579534965496No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151560.7570096818921046No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA148460.7415258470157157No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN118830.5935303543101003No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC114330.5710538198121161No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT112670.562762475975082No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT110280.5508249387639305No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC101720.5080695753633206No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA91710.4580717730689161No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT89950.4492809506874823No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA89520.44713319294656373No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA86270.43090014025357526No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA82720.41316865192738783No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA75410.37665677033177364No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA74640.3728107855398963No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC69880.34903560682647317No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT66020.32975573501264677No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT64990.32461110600533044No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC64330.3213145476122928No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA61310.3062302955714234No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC57340.28640099735875746No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA56950.2844530310355988No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA56170.2805570983892816No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA54540.27241559811556737No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA47280.236153455792153No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA46070.23010976540491726No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA44780.22366649218216184No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT44560.2225676393844826No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC43000.21477577409184812No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA40200.20079037484865803No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA38960.19459684089810236No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA37140.1855063313900288No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT32620.16292990118316478No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA32510.16238047478432516No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA32240.1610318827144461No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG32090.16028266489784665No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC31360.15663647152372923No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG31350.15658652366928927No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA31240.15603709727044965No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA30870.15418902665617096No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA30650.15309017385849172No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC30380.1517415817886127No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG28940.14454909074925779No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA27710.1384055046531421No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA27200.13585816407670392No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA27060.1351588941145444No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT26930.13450957200682487No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT25770.12871562089178898No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG25480.12726713311303No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC25440.12706734169527015No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG23470.1172276143705971No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA23230.11602886586403795No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC22570.11273230747100027No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC22130.11053460187564185No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA21770.1087364791158031No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT21400.10688840850152442No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA21290.10633898210268479No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA21140.10558976428608532No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT21110.10543992072276542No Hit
TGGAGAGAAAGGCAGTTCCTGACGCCTTGGCCGTA20810.1039414850895665No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA20790.10384158938068655No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG20480.10229320589304766No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA20390.10184367520308799No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT20330.10154398807644818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)