FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4570911
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2965986.488815905625815No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT860361.8822506060608049No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA851891.8637203830921232No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA748021.6364790301101904No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA548401.1997608354220854No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN542671.187225041135126No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN500841.0957115550926282No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA310770.679886350882789No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA307890.6735856375238984No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA275450.6026151023286168No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA269170.5888760468099248No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT266420.5828597406512618No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC264830.5793812218177077No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246710.5397392336013543No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA204850.44816011512803466No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT203000.44411278189402503No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT202880.443850252170738No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196280.42941111738994703No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC191450.41884429602764084No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC187630.4104870998363346No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA186000.406921071095018No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT184700.40407699909274103No Hit
CTGACCTATGAATTGACAGCCATAAGGTTAATGCA182320.39887015958088007No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA163250.3571498110551704No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA147460.3226052749659751No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC145110.31746406788493586No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA135570.2965929548836107No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA133250.2915173802333933No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTT122750.26854602944577133No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG117240.2564915396515049No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG116510.2548944838348417No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT114160.24975327675380246No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC112740.24664667502823837No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT110890.24259934179422876No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA106470.23292949698648693No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT105230.2302166898458535No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA103910.22732886288969528No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA100550.21997803063765628No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA93660.20490444902558813No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC90730.19849434828199453No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC89750.1963503555418165No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC86530.18930580796694577No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC83070.1817362009455008No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA82050.17950469829756038No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC80180.1754136101096696No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA79660.17427598130875882No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTATTAC74840.1637310374233933No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA74300.16254965366860127No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG71530.1564895925560572No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69250.15150152781360213No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA69210.15141401790583978No Hit
GGTTCCATAGTGTAGTGGTCGCTTATTCCAATTAC67700.14811051888781035No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA66900.14636032073256294No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA66640.14579150633210752No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT66100.14461012257731556No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT65260.1427724145143058No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC61060.133583874199257No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT60030.13133049407437597No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA58760.12855205450292076No Hit
ACTGGACTTGGAGTCAGAAGGCTATTCCAATTACG58230.12739254822506935No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACC57550.12590487979310908No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC57290.12533606539265366No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC57230.12520480053101013No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT56900.12448284379197058No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC55310.1210043249584164No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG55100.12054489794266394No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG55030.12039175560407979No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC54950.12021673578855507No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54490.11921037184928782No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC54290.11877282231047596No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT53980.1180946205253176No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC51500.11266900624405068No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC50340.110131218918942No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT50210.10984681171871429No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT49680.10868730544086287No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA49400.10807473608652629No Hit
ACTGGACTTGGAGTCAGAAGGCCGCCTTGGCCGTA48640.10641204783904128No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA48170.10538380642283343No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC47010.10284601909772471No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT46100.1008551686961308No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC46060.10076765878836845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)