FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768111
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN548411.455397678040801No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA534241.4177926287203322No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT529771.4059299208542424No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC464201.231917000321912No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA413311.0968625924236308No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA379101.006074396428343No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA329050.8732492222230184No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT321730.8538230428986832No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT320910.8516468861984162No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN287410.7627429234436035No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA271100.7194586359053649No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA259870.689655904510244No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT232650.6174181174599156No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224030.5945419336107668No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT208320.5528499558532113No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA200060.5309291578724724No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170650.45287944012265036No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGAT170480.4524282856847901No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA163190.43308172184948907No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC161770.4293132553685387No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA151180.4012089877394801No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA143970.38207473187493685No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC137030.363657015411701No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT130830.34720314767797444No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA127030.33711851906698076No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA120090.31870080260374495No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC117020.31055348422591583No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC108030.2866953760120124No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA103080.2735588203213759No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC102530.2720992030224163No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG101240.26867573699394737No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA98660.26182880493700955No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA97400.2584849543975748No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA90010.23887300559882657No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG79490.2109545074441809No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC74360.19734025881933945No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC73450.19492525565196991No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC73220.19431487023604135No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGAGGC71810.1905729422514358No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA69500.18444254959580544No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA68880.1827971628224328No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA64310.17066906999289563No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGCTCG62180.16501637027147026No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG59430.15771828377667219No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC58800.1560463585069548No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC56550.15007519682939277No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC55650.14768673215836794No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT55040.14606788388134No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA55040.14606788388134No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC54490.1446082665823804No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT53310.1414767240137034No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA51900.13773479602909786No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC51250.13600979376669103No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC50030.1327720972126352No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA49900.13242709676015382No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA49840.13226786578208552No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT49740.1320024808186383No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC49650.13176363435153582No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC49450.1312328644246414No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT49160.13046324803064452No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT48580.12892401524265076No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG48320.12823401433768802No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA48250.12804824486327498No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA47960.1272786284692781No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC44590.1183351552011074No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC44510.11812284723034963No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC44130.11711438436925027No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA43710.11599976752277201No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT43530.11552207458856704No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA41840.11103706870630933No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA41760.11082476073555159No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT41400.10986937486714166No Hit
CGCCTTGGCCGTACAGCAGGATCATCCTTAGCTTA39880.10583552342274419No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT39610.10511898402143674No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG39540.1049332145470237No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC38280.10158936400758896No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)