FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3591467
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT43592312.137742042457859No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2071565.768005107662134No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC960012.6730302686896468No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT591431.646764400174079No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC493221.3733106833502855No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA423921.1803533208017782No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA412581.1487784796574771No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA289870.8071075134478474No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA287490.8004806949360805No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG271170.7550396537125358No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA218580.6086092396226945No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG193440.5386099886202491No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA151340.42138769477764937No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA150770.41980059958785637No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA146650.40832896418093223No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG143200.39872286171639615No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA141880.39504748338213885No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140740.391873293002553No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT137230.38210012788645975No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATTAC132890.370015929423826No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA114160.31786453836273587No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC105470.29366829766220875No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA103090.28704147915044187No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT102150.28442416427604655No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT101750.28331041326566553No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG96030.26738377381721734No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT83820.23338652422533746No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA76740.21367313134159382No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA74360.20704631282982694No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA69830.19443308263726217No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA69630.19387620713207165No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67740.18861373360802147No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT66330.18468776129642847No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGCGGCAT65540.18248810305092597No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT62420.17380084516995423No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT59070.1644731804580134No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC56400.15703889246372027No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA55440.15436589003880585No Hit
AGCAGCATTGCGTGAAATCGCAGTATTCCATAATG54260.15108032455818193No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA53300.14840732213326754No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC51740.14406369319278167No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG51060.14217031647513398No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA49830.13874553211821242No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT48790.13584977949122182No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC48300.1344854345035051No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47460.13214655738170503No Hit
TGGAATGTAAAGAAGTATGTATCGCCTTGGCCGTA47410.1320073385054074No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT47300.13170105697755263No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTCCGGCA45750.12738527181232626No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGCGGCA45370.12632720835246433No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA43040.11983960871699503No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTTTAATG42550.1184752637292783No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA41230.11479988539502103No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA41060.1143265412156091No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40950.11402025968775434No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC40270.11212688297010664No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC39780.11076253798238993No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC39650.1104005689040161No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39640.11037272512875657No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTAATTACG38070.10600125241301117No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA37440.10424709457166112No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC37310.1038851254932873No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36930.10282706203342533No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA36320.10112859174259431No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGCGGCATG36010.10026543470954906No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)