FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018829
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA672982.22927499371445No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT639832.1194642028415656No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT585791.940454394733852No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT545651.8074889303103951No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC468151.5507668702003325No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT382191.2660206987543845No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA362311.2001673496577647No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC352241.166810044557012No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA283940.9405633773890473No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA282070.9343689225192948No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA278340.9220131382069008No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA262260.8687474514124516No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA257030.8514228530334114No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT245220.8123017236153489No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA233780.7744062350003925No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA223620.7407508010556411No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC200910.665522956086615No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA175540.5814837475060693No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT159610.528714942118285No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA147540.4887325515953372No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA134780.446464506601732No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC129050.4274836368671428No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC127690.4229785787800502No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA124620.41280907265698064No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCTAC116580.38617622925975603No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA114110.377994248763345No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC110010.36441282364784494No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT108430.35917900616431075No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA99620.32999550487954105No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC98270.3255235722195593No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT98200.32529169422978244No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95880.3176065951400361No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT92860.3076027161525214No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG91860.3042901734414238No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG87710.29054312119036885No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC86080.2851436765712798No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT82510.27331789909266146No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA79240.2624858844273723No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCATG78800.2610283656344894No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG78270.2592727179976077No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67270.2228347481755343No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA66970.221840985362205No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC66870.22150973109109523No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA66760.22114535139287456No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC66640.2207478462675428No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA66300.21962158174576962No Hit
CCCAGTGTTCAGACTACCTGTTCGCCTTGGCCGTA63560.21054521471736226No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA62390.20666953974537808No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG62320.20643766175560124No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC60570.2006407120111805No Hit
TCCCTGAGACCCTAACTTGTCGCCTTGGCCGTACA59550.19726191844586094No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT58180.19272373493165726No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG58120.19252498236899143No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC57960.1919949755352158No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC57270.18970932106455848No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT56360.18669490719745968No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA55720.18457487986235724No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC51430.1703640716317486No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC50980.16887342741175468No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT50290.16658777294109736No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA50020.165693386409101No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC49580.16423586761621808No Hit
TCCCTGAGACCCTAACTTGTGCGCCTTGGCCGTAC47420.1570807753602473No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCTTG45730.1514825781784924No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC45240.14985943225005458No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA44730.1481700354673948No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG44640.14787190662339603No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA44300.14674564210162283No Hit
AACCCGTAGATCCGATCTTGTCGCCTTGGCCGTAC44140.14621563526784725No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA43980.14568562843407162No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC43690.14472499104785333No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT42810.14180995346208747No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC41770.13836490904254597No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC38930.12895728774302884No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG38870.12875853518036298No Hit
TTCACAGTGGCTAAGTTCCGACGCCTTGGCCGTAC38860.128725409753252No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA38460.12740039266881298No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA38390.12716851467903614No Hit
CCCAGTGTTCAGACTACCTGTTTCGCCTTGGCCGT37040.12269658201905442No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA36620.12130531408039343No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCTAC35910.11895340875551415No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCAAC35350.1170983848372995No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA35350.1170983848372995No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA34430.11405084554308971No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG34150.1131233335839824No Hit
AACTGGCCCTCAAAGTCCCGCTCGCCTTGGCCGTA33980.11256020132309581No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG33160.1098439162999958No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA33100.10964516373732994No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG31780.10527260735868113No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA31720.10507385479601528No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31240.10348383429468844No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC31060.10288757660669087No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC30820.10209256635602744No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA30770.10192693922047256No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA30670.10159568494936282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)