FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3470899
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1427284.112133484725427No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1235473.5595100865798743No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA794342.288571347077515No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN719682.073468573991925No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA691531.9923656666471712No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT532551.5343287142610602No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT404091.1642228713656029No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN388781.1201132617226832No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT337990.9737822967479031No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC311940.8987296951020471No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT292400.8424330411227754No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT283920.8180013304910342No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT271570.7824197707856091No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN235110.6773749394609293No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA205250.5913453546185009No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA200920.5788702004869631No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196250.5654154730517944No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA194340.5599125759637489No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA190530.5489355927671764No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT178790.5151115028123838No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC170880.49232201801320064No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA161970.4666514352621612No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA160100.46126378209218993No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT151400.43619822991104035No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA140660.4052552379081039No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA132830.38269624094506927No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAT131710.37946941123899025No Hit
CCACTACGCCTTGGCCGTACAGCAGGTCGCAACCT124850.3597050792892562No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA104750.301795010456945No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA102030.29395842402789596No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT99790.2875047646157379No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA96190.27713281198905526No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA94760.2730128419179008No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA90820.2616613159875871No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT86960.25054027789342187No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA81880.23590430029799195No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT81850.23581786735943627No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA79360.22864393345931414No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT71810.20689164392279927No Hit
TGACCTATGAATGTCACTAACATAAGGTTAATGCA70500.20311740560586752No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA70360.20271405189260763No Hit
GCCCGGATAGCTCAGTTGGTAGAGCAGCGCGCCTT70050.20182091152753223No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT69880.2013311248757166No Hit
TAATACTGCCTGGTAATGATGACGCCTTGGCCGTA66210.1907574953924041No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA66160.1906134404948113No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA65190.1878187754815107No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT65040.18738661078873226No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG64390.1855138971200257No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA63300.18237350035250233No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA62700.18064484158138858No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA62130.17900261574883047No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC62070.17882974987171912No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC61830.1781382863632736No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA61360.17678417032590116No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA58790.16937974858963054No Hit
TGTAACAGCAACTCCATGTGGACGCCTTGGGGCAT58400.1682561203884066No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA57860.1667003274944042No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCC54040.1556945333183132No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA54020.15563691135927607No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT53780.15494544785083056No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC53640.1545420941375707No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA53120.15304392320260543No Hit
CGCCTTGGCCGTACAGCAGGTCGCAACCTAGAGAA53050.15284224634597549No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG52620.1516033742266773No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT52550.15140169737004736No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA52500.15125764247245455No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC50960.14682075162659589No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC50850.1465038308518917No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA50590.14575474538440905No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA50080.14428538542896235No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT48800.1405975800505863No Hit
TAACACTGTCTGGTAACGATGTTCGCCTTGGCCGT48770.14051114711203064No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG48400.1394451408698438No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG46420.1337405669251684No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC44940.12947654195642108No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC44930.12944773097690254No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA44420.12797837102145582No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG42700.12302288254426304No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA42170.12149590062977919No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC41990.12097730299844506No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA41640.11996891871529539No Hit
TGTAACAGCAACTCCATGTGGAACGCCTTGGCCGT40560.1168573329272906No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA40230.11590657060317802No Hit
TCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTAC38620.11126800290068942No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG38460.11080702722839242No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA37920.10925123433439003No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA36950.10645656932108945No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35600.10256708708608346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)