FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112721
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1211953.8935388041523797No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT772492.481719370287282No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA758542.436903275301577No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA415351.334363086187294No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN374961.2046052312430187No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT350341.1255104456840173No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311871.0019208274689573No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA311671.0012783028096641No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA296790.9534744681582448No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA238010.7646364707919534No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT229340.7367830268115902No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA206670.6639528566806984No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA198490.6376735981156038No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC176710.5677026627185668No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA175760.5646506705869238No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC165960.5331669622815537No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165420.5314321457014618No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC163030.5237539760229073No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN159630.5128310568149217No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG144130.4630353957196935No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA125050.4017385432231157No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT124990.40154578582532774No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA121360.3898839632591549No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA119470.3838121052288335No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGCTCC115250.3702548349177456No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA102870.3304825585074923No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC98220.31554386017892383No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA96510.3100502743419664No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA90820.29177044778507294No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT89890.2887827081193592No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGGAGG84460.271338163619547No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC83760.26908932731202057No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG77300.24833578081684804No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC71200.22873877870840334No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA69290.22260266821215266No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC67730.21759097586966517No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGCAGG66800.21460323620395147No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT66000.21203313756677838No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGAGAA64770.20808161091212482No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT64200.20625041563313898No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG61640.19802609999418516No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT60290.1936890585439556No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG58530.18803484154217484No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA58190.1869425496213763No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC57910.1860430150983657No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA56580.18177022611406546No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC55580.17855760281759914No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA53210.17094368560497392No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT52850.16978714121824603No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA52570.16888760669523548No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA51740.16622112935916838No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC50840.16332976839234867No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA50390.16188408790893885No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGCTCT50070.1608560484540696No Hit
CCACTACGCCTTGGCCGTACAGCAGGTATCCAACG49520.1590891056410131No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA49410.1587357170784018No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48450.15565159871379414No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGCAGA47010.1510254211668826No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA46930.15076841130316532No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT44680.14354000888611604No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA43610.14010250195889706No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC43470.13965273469739178No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC43060.13833555914584056No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41420.13306685693963577No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG41350.13284197330888314No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC41180.13229582734848386No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA40940.13152479775733192No Hit
CGCCTTGGCCGTACAGCAGGTATCCAACGAGGTCC40790.13104290426286197No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA40370.1296936024783461No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA39040.12542081349404588No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA38720.12439277403917665No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC36960.1187385570373959No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA36210.11632908956504616No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC36170.11620058463318748No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA36080.11591144853650552No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA33920.10897218221613822No Hit
CGCCTTGGCCGTACAGCAGCGCGAACCGGCATGTC33650.10810477392609233No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA32470.10431387843626203No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA32430.10418537350440338No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG32390.10405686857254473No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT32350.10392836364068608No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA32240.10357497507807478No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT32010.10283607171988753No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA31800.10216142082762958No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG31370.10077999281014906No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT31130.10000896321899713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)