FastQCFastQC Report
Wed 8 May 2024
EGAF00002406416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615686
Total Bases126.5 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC44857012.40622111543978No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1516994.195580036540783No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT840542.324704081051286No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA762492.108839097200365No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN541991.4989963177112173No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA488121.3500066100872696No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT473541.3096823120149261No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC452631.2518509627218737No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA429901.188985990487006No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA370711.0252826158023678No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN337340.9329903094461189No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT317040.8768460535566418No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT303460.8392874823754054No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA254810.7047348691230377No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA248490.68725547517124No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA212360.5873297625955352No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC201470.5572109967513772No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA199540.5518731438515402No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT191400.5293601269579272No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT182300.5041920122488512No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC168430.4658313802691937No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154740.4279685791299355No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT152250.42108191917107846No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA147010.4065895102616765No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144160.3987071886220208No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA132250.3657673813489335No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA120350.33285523134475725No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT112760.31186336424125327No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC112400.31086770256045465No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA100980.27928310146400986No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA94150.26039318679774737No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGGCGT93710.2591762669656602No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA88050.24352225276199316No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA84630.23406346679440637No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT81330.22493656805375245No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA75760.2095314692702851No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT73060.20206400666429553No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA66880.18497181447725272No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG65740.18181888582139044No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGGATG63350.17520879855164415No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC57550.1591675825832221No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC57260.158365521784801No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG56710.156844371994692No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC56410.15601465392735983No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG52550.14533894812768586No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC52350.14478580274946443No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA52100.1440943710266876No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG51880.14348591111064402No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA48930.13532701678187764No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT48780.13491215774821155No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT48670.13460792779018974No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47880.13242300354621503No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC47670.1318422008990825No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC47650.13178688636126035No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC46500.12860630043648702No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG46230.12785955417588807No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG46060.12738938060439983No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT45010.12448536736873722No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC44410.12282593123407289No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC43730.12094523694811996No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA43510.12033677703207635No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC43330.11983894619167705No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT43230.11956237350256632No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC43030.11900922812434486No Hit
TGACCTATGAATTGACAGCCGCCTTGGCCGTACAG41710.1153584686280833No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA40940.11322885892193071No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA40690.11253742719915391No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA40650.11242679812350961No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40290.111431136442711No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA39660.10968872850131343No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA39480.10919089766091414No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC39250.10855478047595947No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC39150.10827820778684875No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC38520.10653579984545118No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC38270.10584436812267436No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT37570.10390835929889929No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG37470.10363178660978856No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA36540.10105966060105884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)